1887

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Volume 69, Issue 8

Molecular characterization of a novel -carrying Tn transposon in isolated from swine Free

Abstract

The gene has rarely been reported in strains and its genetic environment has not yet been investigated.

To identify the gene in isolated from swine and characterize its genetic environment.

A isolate (named MM1L5) from a deceased swine was identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and subjected to antimicrobial susceptibility testing. The genes were detected and then the genetic location and environment of were investigated by Southern blot and PCR mapping, respectively. The gene was cloned and expressed in .

Isolate MM1L5 harboured the and genes. The gene, located on the chromosome, was co-carried with an IS and gene by a novel 6361 bp IS-flanked composite transposon, designated Tn. This transposon consisted of a novel -containing module, IS-ΔIS -Δ-IS (named Tn), and a -containing module, IS-Δ -tnpR-IS, differing from previous reports. Phylogenetic analysis showed a significant variation based on the sequence of Tn, as compared to those of other related transposons. Interestingly, although the cloned gene could confer resistance to ceftiofur, cefquinome, ceftriaxone and cefotaxime, one amino acid substitution (Ile-142-Thr) resulted in a significant reduction of resistance to these antimicrobials.

This is the first time that has been identified on a chromosome from a isolate. Furthermore, the gene was located with an IS element and gene on a novel IS-flanked composite transposon, Tn, suggesting that Tn might act as a reservoir for the and genes and may become an important vehicle for their dissemination among .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): blaCTX-M-3 , composite transposon , IS26 and Morganella morganii
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2016YFD05101304)
    • Principle Award Recipient: Gong Zheng Hu
© 2020 The Authors
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2020-07-21
2024-04-18
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Molecular characterization of a novel blaCTX-M-3-carrying Tn6741 transposon in Morganella morganii isolated from swine
J. Med. Microbiol. 69, 1089 (2020); https://doi.org/10.1099/jmm.0.001235
/content/journal/jmm/10.1099/jmm.0.001235
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