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Volume 100, Issue 7

HIV-1 restriction by endogenous APOBEC3G in the myeloid cell line THP-1 Free

Abstract

HIV-1 replication in CD4-positive T lymphocytes requires counteraction of multiple different innate antiviral mechanisms. Macrophage cells are also thought to provide a reservoir for HIV-1 replication but less is known in this cell type about virus restriction and counteraction mechanisms. Many studies have combined to demonstrate roles for APOBEC3D, APOBEC3F, APOBEC3G and APOBEC3H in HIV-1 restriction and mutation in CD4-positive T lymphocytes, whereas the APOBEC enzymes involved in HIV-1 restriction in macrophages have yet to be delineated fully. We show that multiple APOBEC3 genes including APOBEC3G are expressed in myeloid cell lines such as THP-1. Vif-deficient HIV-1 produced from THP-1 is less infectious than Vif-proficient virus, and proviral DNA resulting from such Vif-deficient infections shows strong G to A mutation biases in the dinucleotide motif preferred by APOBEC3G. Moreover, Vif mutant viruses with selective sensitivity to APOBEC3G show Vif null-like infectivity levels and similarly strong APOBEC3G-biased mutation spectra. Importantly, APOBEC3G-null THP-1 cells yield Vif-deficient particles with significantly improved infectivities and proviral DNA with background levels of G to A hypermutation. These studies combine to indicate that APOBEC3G is the main HIV-1 restricting APOBEC3 family member in THP-1 cells.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): APOBEC3G , G to A hypermutation , HIV-1 restriction , myeloid cell line THP-1 and Vif
© 2019 The Authors
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2019-05-30
2024-04-19
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HIV-1 restriction by endogenous APOBEC3G in the myeloid cell line THP-1
J. Gen. Virol. 100, 1140 (2019); https://doi.org/10.1099/jgv.0.001276
/content/journal/jgv/10.1099/jgv.0.001276
/content/journal/jgv/10.1099/jgv.0.001276
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