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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., a novel member of isolated from activated sludge in Hefei, PR China No Access

Abstract

A floc-forming bacterial strain, designated HF-7, was isolated from the activated sludge of an industrial wastewater treatment plant in Hefei, PR China. Cells of this strain were Gram-stain-positive, catalase- and oxidase-negative, facultatively anaerobic, and rod-shaped. Growth occurred at 20–42 °C (optimum, 28 °C), at pH 5.5–10.5 (optimum, pH 7.5) and with 0–8.0 % (w/v) NaCl (optimum, 1 %). The major fatty acid was anteiso-C. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 67 mol% from whole genomic sequence analysis. Based on the results of 16S rRNA gene sequence analysis, this strain should be assigned to the genus and is closely related to CAU 1319 (95.87 % similarity), IPBSL-7 (95.19 %) and Ben 106 (94.63 %) but separated from them by large distances in different phylogenetic trees. Based on whole genome analysis, the orthologous average nucleotide identity and DNA–DNA hybridization values against two of the closest relatives were 75.21–76.50 % and 14.2–24.4 %, respectively. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrated that strain HF-7 could be distinguished from its phylogenetically related species and represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HF-7 (=KCTC 49959=CCTCC AB 2023019).

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Keyword(s): floc formation , novel species , salt tolerance and Tessaracoccus caeni
Funding
This study was supported by the:
  • Key Technologies Research and Development Program (Award 2019YFA0905500)
    • Principle Award Recipient: DongruQiu
© 2023 The Authors
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2023-11-22
2024-04-30
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References

  1. Maszenan AM, Seviour RJ, Patel BK, Schumann P, Rees GN. Tessaracoccus arenae sp. nov., isolated from sea sand. Int J Syst Bacteriol 1999; 49:459–468 [View Article] [PubMed]
     [Google Scholar]
  2. Thongphrom C, Kim JH, Bora N, Kim W. Tessaracoccus arenae sp. nov., isolated from sea sand. Int J Syst Evol Microbiol 2017; 67:2008–2013 [View Article] [PubMed]
     [Google Scholar]
  3. Lee DW, Lee SD. Tessaracoccus flavescens sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2008; 58:785–789 [View Article] [PubMed]
     [Google Scholar]
  4. Srinivasan S, Sundararaman A, Lee SS. Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant. Antonie van Leeuwenhoek 2017; 110:1–9 [View Article] [PubMed]
     [Google Scholar]
  5. Kumari R, Singh P, Schumann P, Lal R. Tessaracoccus flavus sp. nov., isolated from the drainage system of a lindane-producing factory. Int J Syst Evol Microbiol 2016; 66:1862–1868 [View Article] [PubMed]
     [Google Scholar]
  6. Puente-Sánchez F, Sánchez-Román M, Amils R, Parro V. Tessaracoccus lapidicaptus sp. nov., an actinobacterium isolated from the deep subsurface of the Iberian pyrite belt. Int J Syst Evol Microbiol 2014; 64:3546–3552 [View Article] [PubMed]
     [Google Scholar]
  7. Kämpfer P, Lodders N, Warfolomeow I, Busse H-J. Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid. Int J Syst Evol Microbiol 2009; 59:1545–1549 [View Article] [PubMed]
     [Google Scholar]
  8. Tak EJ, Kim HS, Lee J-Y, Kang W, Hyun D-W et al. Tessaracoccus aquimaris sp. nov., isolated from the intestine of a Korean rockfish, Sebastes schlegelii, from a marine aquaculture pond. Int J Syst Evol Microbiol 2018; 68:1065–1072 [View Article] [PubMed]
     [Google Scholar]
  9. Li G-D, Chen X, Li Q-Y, Xu F-J, Qiu S-M et al. Tessaracoccus rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis. Int J Syst Evol Microbiol 2016; 66:922–927 [View Article]
     [Google Scholar]
  10. Schlattmann A, von Lützau K, Kaspar U, Becker K. 'Rothia nasisuis' sp. nov., ‘Dermabacter porcinasus’ sp. nov., ‘Propionibacterium westphaliense’ sp. nov. and ‘Tessaracoccus nasisuum’ sp. nov., isolated from porcine nasal swabs in the Münster region, Germany. New Microbes New Infect 2018; 26:114–117 [View Article]
     [Google Scholar]
  11. Cai M, Wang L, Cai H, Li Y, Wang Y-N et al. Salinarimonas ramus sp. nov. and Tessaracoccus oleiagri sp. nov., isolated from a crude oil-contaminated saline soil. Int J Syst Evol Microbiol 2011; 61:1767–1775 [View Article] [PubMed]
     [Google Scholar]
  12. Fall NS, Lo CI, Fournier P-E, Sokhna C, Raoult D et al. Arcanobacterium ihumii sp. nov., Varibaculum vaginae sp. nov. and Tessaracoccus timonensis sp. nov., isolated from vaginal swabs from healthy Senegalese women. New Microbes New Infect 2019; 31:100585 [View Article]
     [Google Scholar]
  13. Meng YC, Liu HC, Zhou YG, Cai M, Kang Y. Vibrio gangliei sp. nov., a novel member of Vibrionaceae isolated from sawdust in a pigpen. Int J Syst Evol Microbiol 2018; 68:1969–1974 [View Article] [PubMed]
     [Google Scholar]
  14. Zhou LY, Zhang JY, Chen XY, Du ZJ, Mu DS. Tessaracoccus antarcticus sp. nov., a rhodopsin-containing bacterium from an Antarctic environment and emended description of the genus Tessaracoccus. Int J Syst Evol Microbiol 2020; 70:1555–1561 [View Article] [PubMed]
     [Google Scholar]
  15. Zhang X-Q, Sun C, Wang C-S, Zhang X, Zhou X et al. Sinimarinibacterium flocculans gen. nov., sp. nov., a gammaproteobacterium from offshore surface seawater. Int J Syst Evol Microbiol 2015; 65:3541–3546 [View Article] [PubMed]
     [Google Scholar]
  16. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology Wiley; 2007 pp 330–393 [View Article]
     [Google Scholar]
  17. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  18. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article] [PubMed]
     [Google Scholar]
  19. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  20. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  21. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  22. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
     [Google Scholar]
  23. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article] [PubMed]
     [Google Scholar]
  24. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
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Tessaracoccus caeni sp. nov., a novel member of Propionibacteriaceae isolated from activated sludge in Hefei, PR China
Int J Syst Evol Microbiol 73, 006113 (2023); https://doi.org/10.1099/ijsem.0.006113
/content/journal/ijsem/10.1099/ijsem.0.006113
/content/journal/ijsem/10.1099/ijsem.0.006113
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