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Volume 71, Issue 12

gen. nov., sp. nov., a member of the family isolated from seawater in East China Sea No Access

Abstract

An aerobic Gram-stain-negative, curved rod-shaped and non-spore-forming bacterial strain (NBU2194) was isolated from seawater collected in an intertidal zone in Ningbo, Zhejiang Province, PR China. It was motile though a single polar flagellum and grew at 20–42 °C (optimum, 30 °C), in 0–2.0 % NaCl (0 %, w/v) and at pH 5.0–9.0 (pH 6.0–7.0). The sole respiratory quinone was ubiquinone-8. The major cellular fatty acids were C, C 7 and/or C 6. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminophosphoglycolipids. A phylogenetic analysis based on 16S rRNA gene sequences and 65 genomic core genes showed that strain NBU2194 formed a distinct lineage in the family . The genome of strain NBU2194 was 4 913 533 bp with a DNA G+C content of 43.9 mol% and coded 3895 genes, 12 rRNA genes and 47 tRNA genes. The average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values between strain NBU2194 and related species of were below the threshold limit for prokaryotic species delineation. NBU2194 could be distinguished from other genera in the family based on phenotypic, chemotaxonomic and genomic characteristics. On the basis of the polyphasic taxonomic evidence collected in this study, strain NBU2194 is considered to represent a novel genus and species in the family , for which the name is proposed. The type strain is NBU2194 (=KCTC 82442=GDMCC 1.2217).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alteromonadaceae , Paraneptunicella aestuarii and taxonomy
Funding
This study was supported by the:
  • the Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund of Ningbo University
    • Principle Award Recipient: NotApplicable
  • the National 111 Project of China (Award D16013)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation (Award 41776168)
    • Principle Award Recipient: ShanHe
  • the State Key Laboratory of Natural and Biomimetic Drugs (Award K202006)
    • Principle Award Recipient: LiweiLiu
  • Ningbo University (Award 422010882,422110473)
    • Principle Award Recipient: LiweiLiu
© 2021 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005160
2021-12-16
2024-04-24
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Paraneptunicella aestuarii gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from seawater in East China Sea
Int J Syst Evol Microbiol 71, 005160 (2021); https://doi.org/10.1099/ijsem.0.005160
/content/journal/ijsem/10.1099/ijsem.0.005160
/content/journal/ijsem/10.1099/ijsem.0.005160
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