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Volume 71, Issue 3

gen. nov. sp. nov., a member of the family with alginate-degradation activity, isolated from marine alga Free

Abstract

A Gram-stain-negative, aerobic, yellow, non-motile, rod-shaped and alginate-degrading bacterium, designated Dm15, was isolated from marine alga collected in Weihai, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Dm15 represents a distinct line of the family . Strain Dm15 had the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour (96.7 %) and 93.7–96.4 % sequence similarity to other phylogenetic neighbours (, , , and ) in the family . The novel isolate was able to grow at 10–40 °C (optimum, 30–33 °C), pH 7.0–9.0 (optimum, pH 7.0–7.5) and with 0.5–6.0 % NaCl (optimum 2.0–3.0 %, w/v). It could grow at 40 °C, and degrade alginate and cellulose, which were different from the neighbour genera. The draft genome consisted of 3395 genes with a total length of 3 798 431 bp and 34.1mol% G+C content. Especially, there were some specific genes coding for cellulase and alginate lyase, which provided a basis for the above phenotypic characteristics. The strain's genome sequence showed 71.1–80.2 % average amino acid identity values and 71.8–77.7 % average nucleotide identity values compared to the type strains of related genera within the family . It shared digital DNA–DNA hybridization identity of 19.8 and 20.9 % with and , respectively. The sole menaquinone was MK-6. The major fatty acids were iso-C and iso-C G. The polar lipids included six unidentified polar lipids, four unidentified aminolipids and phosphatidylethanolamine. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain Dm15 represents a novel species of a new genus in the family , phylum , for which the name gen. nov., sp. nov. is proposed. The type strain is Dm15 (KCTC 42256=CICC 23815).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , Flavihalobacter algicola and polyphasic taxonomy
Funding
This study was supported by the:
  • Key Science and Technology Program of Weihai (Award 1070413421511)
    • Principle Award Recipient: Yan-XiaZhou
  • China Postdoctoral Science Foundation (Award 2183)
    • Principle Award Recipient: Yan-XiaZhou
  • Natural Science Foundation of Shandong Province (Award ZR2017MC019)
    • Principle Award Recipient: Yan-XiaZhou
  • National Natural Science Foundation of China (Award 31700116)
    • Principle Award Recipient: Yan-XiaZhou
© 2021 The Authors
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2021-02-10
2024-04-24
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Flavihalobacter algicola gen. nov. sp. nov., a member of the family Flavobacteriaceae with alginate-degradation activity, isolated from marine alga Saccharina japonica
Int J Syst Evol Microbiol 71, 004701 (2021); https://doi.org/10.1099/ijsem.0.004701
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