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Volume 70, Issue 3

sp. nov., a novel lactic acid bacterium isolated from lychee Free

Abstract

A novel lactic acid bacterium, strain MB7, was isolated from lychee in Taiwan. MB7 is Gram-staining-positive, catalase-negative, non-motile, non-haemolytic, facultatively anaerobic, coccoid-shaped, heterofermentative and mainly produces -lactic acid from glucose. Comparative analysis of 16S rRNA, and gene sequences has demonstrated that the novel strain represented a member of the genus . 16S rRNA gene sequencing results indicated that MB7 had the same sequence similarity of 99.25 % to four type strains of members of the genus : subsp. DSM 20484, subsp. DRC 1506, subsp. ATCC 8293 and DSM 20241. Additionally, high 16S rRNA sequence similarities were also observed with subsp. ATCC 19254 (99.12 %) and NRIC 1777 (98.69 %). When comparing the genomes of these type strains, the average nucleotide identity values and digital DNA–DNA hybridization values of MB7 with these type strains were 76.57–80.53 and 22.0–22.6 %, respectively. MB7 also showed different phenotypic characteristics to other most closely related species of the genus , such as carbohydrate metabolizing ability, halotolerance and growth at various pHs. On the basis of phenotypic and genotypic properties, strain MB7 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is MB7 (=BCRC 81077=NBRC 113542).

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  • Published Online:
Keyword(s): Lactic acid bacteria , Leuconostoc sp. nov. , lychee and Taiwan
© 2020 The Authors
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2020-01-02
2024-04-16
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References

  1. Jeon HH, Kim KH, Chun BH, Ryu BH, Han NS et al. A proposal of Leuconostoc mesenteroides subsp. jonggajibkimchii subsp. nov. and reclassification of Leuconostoc mesenteroides subsp. suionicum (GU et al., 2012) as Leuconostoc suionicum sp. nov. based on complete genome sequences. Int J Syst Evol Microbiol 2017; 67:2225–2230 [View Article]
     [Google Scholar]
  2. Chen YS, Liao YJ, Lan YS, Wu HC, Yanagida F. Diversity of lactic acid bacteria associated with banana fruits in Taiwan. Curr Microbiol 2017; 74:484–490 [View Article]
     [Google Scholar]
  3. Chen YS, CR Y, Chen ZY, YC L, HC W. Isolation and characterization of lactic acid bacteria from xi-gua-mian (fermented watermelon), a traditional fermented food in Taiwan. Ital J Food Sci 2016; 28:9–14
     [Google Scholar]
  4. Chen YS, Wu HC, Yanagida F, Chen YS. Isolation and characteristics of lactic acid bacteria isolated from ripe mulberries in Taiwan. Braz J Microbiol 2010; 41:916–921 [View Article]
     [Google Scholar]
  5. Leong KH, Chen YS, Pan SF, Chen JJ, Wu HC et al. Diversity of lactic acid bacteria associated with fresh coffee cherries in Taiwan. Curr Microbiol 2014; 68:440–447 [View Article]
     [Google Scholar]
  6. Lyhs U, Snauwaert I, Pihlajaviita S, De Vuyst L, Vandamme P et al. Leuconostoc rapi sp. nov., isolated from sous-vide-cooked rutabaga. Int J Syst Evol Microbiol 2015; 65:2586–2590 [View Article]
     [Google Scholar]
  7. Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P et al. Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 2005; 151:2141–2150 [View Article]
     [Google Scholar]
  8. Chang CH, Chen YS, Lee TT, Chang YC, Yu B et al. Lactobacillus formosensis sp. nov., a lactic acid bacterium isolated from fermented soybean meal. Int J Syst Evol Microbiol 2015; 65:101–106 [View Article]
     [Google Scholar]
  9. Sato H, Yanagida F, Shinohara T, Yokotsuka K. Restriction fragment length polymorphism analysis of 16S rRNA genes in lactic acid bacteria isolated from red wine. J Biosci Bioeng 2000; 90:335–337 [View Article]
     [Google Scholar]
  10. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article]
     [Google Scholar]
  11. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article]
     [Google Scholar]
  12. Chor B, Hendy MD, Snir S. Maximum likelihood Jukes–Cantor triplets: Analytic solutions. Mol Biol Evol 2006; 23:626–632 [View Article]
     [Google Scholar]
  13. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992; 9:945–967
     [Google Scholar]
  14. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526
     [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
     [Google Scholar]
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  17. Klimke W, Agarwala R, Badretdin A, Chetvernin S, Ciufo S et al. The National Center for Biotechnology Information's protein clusters database. Nucleic Acids Res 2009; 37:D216–D223 [View Article]
     [Google Scholar]
  18. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article]
     [Google Scholar]
  19. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
     [Google Scholar]
  20. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article]
     [Google Scholar]
  21. Rosselló-Móra R, Amann R. Past and future species definitions for bacteria and archaea. Syst Appl Microbiol 2015; 38:209–216 [View Article]
     [Google Scholar]
  22. Guu JR, Wang LT, Hamada M, Wang C, Lin RW et al. Lactobacillus bambusae sp. nov., isolated from traditional fermented ma bamboo shoots in Taiwan. Int J Syst Evol Microbiol 2018; 68:2424–2430 [View Article]
     [Google Scholar]
  23. Kozaki M, Uchimura T, Okada S. Experimental Manual of Lactic Acid Bacteria Tokyo: Asakurasyoten; 1992 pp 29–72
     [Google Scholar]
  24. Hyronimus B, Le Marrec C, Sassi AH, Deschamps A. Acid and bile tolerance of spore-forming lactic acid bacteria. Int J Food Microbiol 2000; 61:193–197 [View Article]
     [Google Scholar]
  25. Kandler O, Weiss N. Genus Lactobacillus Beijerinck 1901, 212AL. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriology 2 Baltimore: Williams & Wilkins; 1986 pp 1209–1234
     [Google Scholar]
  26. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . J Antibiot 2012; 65:427–431 [View Article]
     [Google Scholar]
  27. Nozawa Y, Sakai N, Arai K, Kawasaki Y, Harada K. Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey's method. J Microbiol Methods 2007; 70:306–311 [View Article]
     [Google Scholar]
  28. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20:16
     [Google Scholar]
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Leuconostoc litchii sp. nov., a novel lactic acid bacterium isolated from lychee
Int J Syst Evol Microbiol 70, 1585 (2020); https://doi.org/10.1099/ijsem.0.003938
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