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Volume 70, Issue 2

sp. nov., isolated from coastal sediment Free

Abstract

A Gram-stain-negative, cocci or short rod-shaped, yellow-pigmented bacterium, designated strain S2-4-2, was isolated from coastal sediment of in Quanzhou Bay, PR China. Growth was observed at 15–40 °C (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). Strain S2-4-2 tolerated 0–10 % NaCl (optimum, 1 %). The 16S rRNA gene of strain S2-4-2 showed highest sequence similarity to Ery9 (98.2 %), followed by PQ-2, E4A9 and Ery22 (97.6%, 96.3 and 96.3 %, respectively). Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on 92 up-to-date bacterial core gene sets indicated that strain S2-4-2 forms a distinct monophyletic branch affiliated to the genus . The average nucleotide identity value between strain S2-4-2 and its close relatives were estimated to be 74.8–85.7 %. The respiratory quinone was found to be Q-10. The predominant fatty acids (>10 %) were identified as summed feature 8 (Cω7 and/or Cω6) and summed feature 3 (Cω7 and Cω6). The polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and an unidentified phospholipid. The draft genome size of strain S2-4-2 was 3.5 Mb with a genomic G+C content of 63.0 mol%. Based on these results, strain S2-4-2 is concluded to represent a novel species within the genus , for which the name sp. nov. is proposed with the type strain S2-4-2 (=MCCC 1K03706=KCTC 72146).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): coastal sediment , Croceicoccus sediminis and polyphasic taxonomy
Funding
This study was supported by the:
  • National Science Foundation of China (Award No. 31772197)
    • Principle Award Recipient: Jianjun Yuan
  • Funding program for innovation and entrepreneurship for Quanzhou high-level talents (Award No. 2017Z034)
    • Principle Award Recipient: Jianjun Yuan
© 2020 IUMS
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2019-11-04
2024-04-24
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Croceicoccus sediminis sp. nov., isolated from coastal sediment
Int J Syst Evol Microbiol 70, 779 (2020); https://doi.org/10.1099/ijsem.0.003823
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