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Volume 69, Issue 8

gen. nov., sp. nov., a novel member of the family isolated from the Godavari River, India Free

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, oxidase-positive and rod-shaped bacterium, designated PRB40, was isolated from the Godavari River in India during the course of ‘Kumbh Mela’, the world’s largest mass gathering event. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRB40 formed a lineage within the family and was distinct from the most closely related genera , and with sequence similarity values ≤95.2 %. Growth of strain PRB40 occurred at 10–40 °C (optimum 30 °C), at pH 6.0–9.0 (pH 7.0) and with 0–0.5 % (w/v) NaCl concentration (0 %). The major respiratory quinone was ubiquinone-10 (Q-10). It contained Cω6, C 2-OH, summed feature 3 (Cω7 and/or Cω6) and summed feature 8 (C 7 and/or C 6) as the major cellular fatty acids. The predominant polar lipids were phospholipid, phosphatidylethanolamine and sphingoglycolipid. It took -homospermidine as the major polyamine. The DNA G+C content based on its draft genome sequence was 63.7 mol%. The polyphasic taxonomic analyses indicated that strain PRB40 represents a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is PRB40 (=MCC 3406=GDMCC 1.1197=KCTC 52678=LMG 29985).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , FAME , Godavari river , mass bathing , phospholipid and sym-homospermidine
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2019-08-01
2024-04-16
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Chakrabartia godavariana gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from the Godavari River, India
Int J Syst Evol Microbiol 69, 2452 (2019); https://doi.org/10.1099/ijsem.0.003512
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