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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 7

Jannaschia formosa sp. nov., isolated from marine saltern sediment Free

Abstract

A novel blush-red-pigmented, Gram-stain-negative, gliding, aerobic and rod- or oval-shaped bacterium, designated strain 12N15, was isolated from sediment sampled at a marine saltern located in Wendeng, China (36° 59′ 56.49″ N, 122° 1′ 38.84″ E). Growth was observed at 10–40 °C (optimum, 28 °C), in 1.0–12.0 % NaCl (2.0–5.0 %, w/v) and at pH 6.0–9.5 (pH 7.0). The respiratory quinones were determined to be Q-10 and major fatty acids were C18 : 1ω7c and C18 : 0. The polar lipids profile of strain 12N15 included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminolipid, phosphatidylcholine, one lipid and three phospholipids. The genomic DNA G+C content was 69.6 mol%. Phylogenetic analyses based on the 16S rRNA gene showed that the strain 12N15 was affiliated within the genus Jannaschia , and was most closely related to Jannaschia seohaensis KCTC 22172. The average amino acid identity and percentage of conserved protein values between strain 12N15 and the type strain of the type species, Jannaschia helgolandensis DSM 14858, were 70.2 % and 64.1 %, respectively. The average nucleotide identity value between strain 12N15 and J.annaschia seohaensis KCTC 22172 was 81.9 %. The phenotypic, phylogenetic and genomic analyses supported the hypothesis that strain 12N15 represents a novel species of the genus Jannaschia , for which the name Jannaschia formosa sp. nov. is proposed. The type strain is 12N15 (=MCCC 1H00325=KCTC 62582).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Jannaschia and polyphasic taxonomy
© 2019 IUMS
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2019-05-08
2024-04-19
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References

  1. Wagner-Döbler I, Rheims H, Felske A, Pukall R, Tindall BJ. Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 2003; 53:731–738 [View Article][PubMed]
     [Google Scholar]
  2. MacIán MC, Arahal DR, Garay E, Ludwig W, Schleifer KH et al. Jannaschia rubra sp. nov., a red-pigmented bacterium isolated from sea water. Int J Syst Evol Microbiol 2005; 55:649–653 [View Article][PubMed]
     [Google Scholar]
  3. Choi DH, Yi H, Chun J, Cho BC. Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 2006; 56:45–49 [View Article][PubMed]
     [Google Scholar]
  4. Yoon JH, Kang SJ, Park S, Oh TK. Jannaschia donghaensis sp. nov., isolated from seawater of the East Sea, Korea. Int J Syst Evol Microbiol 2007; 57:2132–2136 [View Article][PubMed]
     [Google Scholar]
  5. Kim BY, Yoo SH, Weon HY, Jeon YA, Hong SB et al. Jannaschia pohangensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 2008; 58:496–499 [View Article][PubMed]
     [Google Scholar]
  6. Yoon JH, Kang SJ, Park S, Oh KH, Oh TK. Jannaschia seohaensis sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2010; 60:191–195 [View Article][PubMed]
     [Google Scholar]
  7. Park S, Yoon JH. Jannaschia aquimarina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62:2631–2636 [View Article][PubMed]
     [Google Scholar]
  8. Jung YT, Yoon JH. Jannaschia faecimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:945–951 [View Article][PubMed]
     [Google Scholar]
  9. Park S, Choi SJ, Won SM, Yoon JH. Jannaschia confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2018; 68:669–674 [View Article][PubMed]
     [Google Scholar]
  10. Adachi M, Kanno T, Okamoto R, Shinozaki A, Fujikawa-Adachi K et al. Jannaschia cystaugens sp. nov., an Alexandrium (Dinophyceae) cyst formation-promoting bacterium from Hiroshima Bay, Japan. Int J Syst Evol Microbiol 2004; 54:1687–1692 [View Article][PubMed]
     [Google Scholar]
  11. Pujalte MJ, MacIán MC, Arahal DR, Garay E. Thalassobacter stenotrophicus MacIán et al, 2005 is a later synonym of Jannaschia cystaugens Adachi, et al. 2004, with emended description of the genus Thalassobacter . Int J Syst Evol Microbiol 2005; 25:1959–1963
     [Google Scholar]
  12. Salka I, Moulisová V, Koblízek M, Jost G, Jürgens K et al. Abundance, depth distribution and composition of aerobic bacteriochlorophyll a-producing bacteria in four basins of the central Baltic Sea. Appl Environ Microbiol 2008; 74:4398–4404 [View Article][PubMed]
     [Google Scholar]
  13. Fang H, Cai L, Yang Y, Ju F, Li X et al. Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments. Sci Total Environ 2014; 470-471:983–992 [View Article][PubMed]
     [Google Scholar]
  14. Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:230 [View Article][PubMed]
     [Google Scholar]
  15. Williams ST, Davies FL. Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. Microbiology 1965; 38:251–261 [View Article][PubMed]
     [Google Scholar]
  16. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
     [Google Scholar]
  17. Yoon SH, Sm H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617
     [Google Scholar]
  18. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [View Article]
     [Google Scholar]
  19. Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196:2210–2215 [View Article][PubMed]
     [Google Scholar]
  20. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  21. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  22. Rodriguez-R LM, Konstantinidis KT. Bypassing cultivation to identify bacterial species. Microbe Magazine 2014; 9:111–118 [View Article]
     [Google Scholar]
  23. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  25. Claus D. A standardized gram staining procedure. World J Microbiol Biotechnol 1992; 8:451–452 [View Article][PubMed]
     [Google Scholar]
  26. Bernadet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070
     [Google Scholar]
  27. Wang NN, Li CM, Li YX, Du ZJ. Aquimarina celericrescens sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2018; 68:1683–1688 [View Article][PubMed]
     [Google Scholar]
  28. Zj D, Wang ZJ, Zhao JX, Chen GJ. Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales and proposal of Woeseiaceae fam. nov. Int J Syst Evol Microbiol 2016; 66:107–112
     [Google Scholar]
  29. Zj D, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696
     [Google Scholar]
  30. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
     [Google Scholar]
  31. Fang DB, Han JR, Liu Y, Du ZJ. Seonamhaeicola marinus sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 2017; 67:4857–4861 [View Article][PubMed]
     [Google Scholar]
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Jannaschia formosa sp. nov., isolated from marine saltern sediment
Int J Syst Evol Microbiol 69, 2037 (2019); https://doi.org/10.1099/ijsem.0.003424
/content/journal/ijsem/10.1099/ijsem.0.003424
/content/journal/ijsem/10.1099/ijsem.0.003424
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