1887

Abstract

Three strains of a novel species of ascomycetous yeast were isolated from the beetle species (Scarabaeoidea) collected from the Baotianman and Funiu Mountains of China. These strains produced conjugated asci with a single coiled ascospore. Phylogenetic analysis of the combined sequences of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions demonstrated that the three strains were closely related to and an undescribed yeast strain, 13Y231. The novel strains could be differentiated from CBS 12562 by a 1.6 % sequence divergence (9 substitutions) and from the undescribed yeast strain, 13Y231, by a 1.1 % sequence divergence (6 substitutions) in the D1/D2 sequences. The ITS sequences of these strains displayed more than 4.1 % sequence divergence (12–22 substitutions and 7–8 gaps) from their two closest relatives. Interestingly, all the three strains could ferment -xylose to ethanol effectively, a rare property among members of the clade. Therefore, a novel yeast species, sp. nov., is proposed to accommodate these strains. The type strain of sp. nov. is NYNU 14914 (=CICC 33059=CBS 13927). The MycoBank number is MB 816795.

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2016-10-01
2024-03-28
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and PSI-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Cadete R. M., Santos R. O., Melo M. A., Mouro A., Gonçalves D. L., Stambuk B. U., Gomes F. C., Lachance M. A., Rosa C. A. 2009; Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 9:1338–1342 [View Article][PubMed]
    [Google Scholar]
  3. Daniel H. M., Vrancken G., Takrama J. F., Camu N., De Vos P., De Vuyst L. 2009; Yeast diversity of Ghanaian cocoa bean heap fermentations. FEMS Yeast Res 99:774–783 [View Article][PubMed]
    [Google Scholar]
  4. Daniel H. M., Lachance M. A., Kurtzman C. P. 2014; On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie Van Leeuwenhoek 106:67–84 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Gouliamova D. E., Dimitrov R. A., Smith M. Th., Groenewald M., Stoilova-Disheva M. M., Guéorguiev B. V., Boekhout T. 2016; DNA barcoding revealed Nematodospora valgi gen. nov., sp. nov. and Candida cetoniae sp. nov. in the Lodderomyces clade. Fungal Biol 120:179–190 [View Article][PubMed]
    [Google Scholar]
  7. Ji Z. H., Jia J. H., Bai F. Y. 2009; Four novel Candida species in the Candida albicans/Lodderomyces elongisporus clade isolated from the gut of flower beetles. Antonie Van Leeuwenhoek 95:23–32 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371[PubMed] [CrossRef]
    [Google Scholar]
  10. Kurtzman C. P. 2011; Lodderomyces van der Walt (1971). In The yeasts, a taxonomic study, 5th edn. vol. 2, pp. 561–563 . Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  11. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, A Taxonomic Study, 5th Edn. vol. 1 pp. 87–110 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  12. Lachance M. A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P. 2011; Candida Berkhout (1923). In The Yeasts, a Taxonomic Study, 5th edn. , vol. 2 pp. 987––1278 . Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  13. Lin Y. P., He P., Wang Q. H., Lu D. J., Li Z. L., Wu C. S., Jiang N. T. 2010; The alcohol dehydrogenase system in the xylose-fermenting yeast Candida maltosa . PLoS One 5:e11752 [View Article][PubMed]
    [Google Scholar]
  14. Lohmeier-Vogel E., Skoog K., Vogel H., Hahn-Hägerdal B. 1989; 31P nuclear magnetic resonance study of the effect of azide on xylose fermentation by Candida tropicalis . Appl Environ Microbiol 55:1974–1980[PubMed]
    [Google Scholar]
  15. Nguyen N. H., Suh S. O., Blackwell M. 2007; Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia 99:842–858 [View Article][PubMed]
    [Google Scholar]
  16. Nitiyon S., Boonmak C., Am-In S., Jindamorakot S., Kawasaki H., Yongmanitchai W., Limtong S. 2011; Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand. Int J Syst Evol Microbiol 61:462–468 [View Article][PubMed]
    [Google Scholar]
  17. Ren Y. C., Xu L. L., Zhang L., Hui F. L. 2015; Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles. Int J Syst Evol Microbiol 65:3580–3585 [View Article][PubMed]
    [Google Scholar]
  18. Suh S. O., Nguyen N. H., Blackwell M. 2008; Yeasts isolated from plant-associated beetles and other insects: seven novel Candida species near Candida albicans . FEMS Yeast Res 8:88–102 [View Article][PubMed]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. J. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  21. Urbina H., Schuster J., Blackwell M. 2013; The gut of Guatemalan passalid beetles: a habitat colonized by cellobiose- and xylose-fermenting yeasts. Fungal Ecol 6:339–355 [View Article]
    [Google Scholar]
  22. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications , pp. 315–322 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. New York: Academic Press;
    [Google Scholar]
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