1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 8

sp. nov., a mesophilic, pigmented bacterium isolated from soil Free

Abstract

A pink- to light-reddish-pigmented, aerobic, pointed, budding, mesophilic strain designated MSL-20 was isolated from soil from Bigeum island, Korea. The isolate was Gram-negative. Cells were motile rods without flagella. The temperature and pH for growth were 20–37 °C (optimum 28 °C) and pH 7.0–11.0 (optimum pH 7.0±0.2). Q-10 was the predominant respiratory ubiquinone. The major fatty acids were C (37 %), C 7 (36 %) and 11-methyl C 7 (12 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that DSM 16684 was its closest neighbour (95.2 % similarity); the isolate had less than 97.0 % similarity to all species with validly published names. Chemotaxonomic data were determined for this isolate, including peptidoglycan type, diagnostic sugar composition, fatty acid profiles, ubiquinones and polar lipids; the results supported the affiliation of strain MSL-20 to the genus . The DNA G+C content was 72.3 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain MSL-20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MSL-20 (=KCTC 22012 =DSM 19309).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65590-0
2008-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1797.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65590-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J.(1996). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef] [Google Scholar]
  2. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  3. Denner, E. B. M., Kolari, M., Hoornstra, D., Tsitko, I., Kämpfer, P., Busse, H.-J. & Salkinoja-Salonen, M.(2006).Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderately thermophilic bacterium isolated from coloured slime deposits in paper machines. Int J Syst Evol Microbiol 56, 1355–1362.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Garrity, G. M., Bell, J. A. & Lilburn, T.(2005). Family I. Rhodobacteraceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, p. 161. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  7. Goodfellow, M.(1971). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69, 33–80.[CrossRef] [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  9. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  10. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  11. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  12. Lechevalier, M. P., Stern, A. E. & Lechevalier, H. A.(1981). Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt I (Suppl. 11), 111–116. [Google Scholar]
  13. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and distinct actinomycetes lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  16. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  18. Tsukamura, M.(1966). Adansonian classification of mycobacteria. J Gen Microbiol 45, 253–273.[CrossRef] [Google Scholar]
  19. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65590-0
Loading
Rubellimicrobium mesophilum sp. nov., a mesophilic, pigmented bacterium isolated from soil
Int J Syst Evol Microbiol 58, 1797 (2008); https://doi.org/10.1099/ijs.0.65590-0
/content/journal/ijsem/10.1099/ijs.0.65590-0
/content/journal/ijsem/10.1099/ijs.0.65590-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error