1887

Abstract

In the course of screening novel secondary metabolites, an acidophilic actinomycete strain, designated Aac-30, was isolated from forest soil and subjected to a polyphasic taxonomic characterization study. It grew well on media in which the pH ranged from 4 to 6, but not on medium with pH adjusted to 7. It possessed -diaminopimelic acid and glycine in the cell-wall peptidoglycan, MK-9(H) and MK-9(H) as major isoprenoid quinones, iso-C and anteiso-C as major cellular fatty acids, and phosphatidylglycerol and phosphatidylinositol as polar lipids. The G+C content of the genomic DNA was 69.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism belonged to the family and consistently formed a monophyletic cluster with members of the genus . Physiological, biochemical and chemotaxonomic data revealed that this novel organism could be readily differentiated from recognized members of the genus and that it merits separate species status. Based on the phenotypic and genetic evidence presented, strain Aac-30 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Aac-30 (=NBRC 101179=DSM 44948).

Erratum

An erratum has been published for this content:
sp. nov., an acidophilic actinomycete isolated from forest soil
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65056-0
2007-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2272.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65056-0&mimeType=html&fmt=ahah

References

  1. Busti E., Cavaletti L., Monciardini P., Schumann P., Rohde M., Sosio M., Donadio S. 2006; Catenulispora acidiphila gen. nov., sp. nov. a novel, mycelium-forming actinomycete, and proposal of Catenulisporaceae fam. nov. Int J Syst Evol Microbiol 56:1741–1746 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. 1988; Simple genetic identification method of viridans group streptococci by colorimetric dot hybridization and quantitative fluorometric hybridization in microdilution wells. J Clin Microbiol 26:1708–1713
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  5. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  6. Rayner R. W. 1970 A Mycological Colour Chart Kew, UK: Commonwealth Mycological Institute and British Mycological Society;
    [Google Scholar]
  7. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  8. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  9. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  10. Seino A., Arai M., Enokita R., Okazaki T., Furuichi A. 1985; Identification Manual of Actinomycetes . Tokyo: The Society; for Actinomycetes Japan (in Japanese
  11. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  12. Tamura T., Hatano K. 2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘ Actinoplanes aurantiacus ’ to Cryptosporangium minutisporangium comb.nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:2119–2125 [CrossRef]
    [Google Scholar]
  13. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994 A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203 [CrossRef]
  14. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev. a new genus of the order Actinomycetales . Int J Syst Bacteriol 43805–812 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65056-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65056-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error