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Volume 57, Issue 11

gen. nov., sp. nov., a novel actinomycete of the family Free

Abstract

A Gram-positive, non-motile, non-mycelium-forming, rod-shaped actinomycete, designated KSW2-17, was isolated from dried seaweed collected from beach sand along the coast of Jeju, Republic of Korea. The organism had ornithine as the diagnostic cell-wall diamino acid, MK-10 and MK-11 as the major menaquinones, and phosphatidylglycerol and diphosphatidylglycerol as polar lipids. The fatty acid profile included predominantly iso- and anteiso-branched acids and a minor amount of tuberculostearic acid (10-methyl C). The DNA G+C content was 68.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the seaweed isolate formed a distinct clade within the radiation of the family and had highest sequence similarity (96.1–96.3 %) to members of the genera , and . On the basis of phenotypic and genotypic evidence, strain KSW2-17 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is KSW2-17 (=JCM 14008=KCTC 19176).

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2007-11-01
2024-04-26
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References

  1. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. 2002; Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; a polyphasic characterization of Subtercola pratensis sp.nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov.,sp. nov. Int J Syst Evol Microbiol 52:1441–1454 [CrossRef]
     [Google Scholar]
  2. Evtushenko L. I., Takeuchi M. 2003; The family Microbacteriaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria . , 3rd edn. vol 3 pp 1020–1098 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  3. Evtushenko L. I., Dorofeeva L. V., Dobrovolskaya T. G., Streshinskaya G. M., Subbotin S. A., Tiedje J. M. 2001; Agreia bicolorata gen. nov., sp. nov. to accommodate actinobacteria isolated from narrow reed grass infected by the nematode Heteroanguina graminophila . Int J Syst Evol Microbiol 51:2073–2079 [CrossRef]
     [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
     [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  6. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
     [Google Scholar]
  7. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp. nov., P.filamentus sp. nov., gas vacuolated polar marine bacteria of the Cytophaga – Flavobacterium – Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
     [Google Scholar]
  8. Han S. K., Nedashkovskaya O. I., Mikhailov V. V., Kim S. B., Bae K. S. 2003; Salinibacterium amurskyense gen. nov., sp. nov. a novel genus of the family Microbacteriaceae from the marine environment. Int J Syst Evol Microbiol 53:2061–2066 [CrossRef]
     [Google Scholar]
  9. Hancock I. C. 1994; Analysis of cell wall constituents of Gram-positive bacteria. In Chemical Methods in Prokaryotic Systematics pp 63–84 Edited by Goodfellow M., O'Donnell A. G. Chichester: John Wiley & Sons;
     [Google Scholar]
  10. Hopwood D. A., Bibb M. J., Chater K. F., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985; Genetic Manipulation of Streptomyces. A Laboratory Manual . Norwich: John Innes Foundation;
     [Google Scholar]
  11. Inoue K., Komagata K. 1976; Taxonomic study on obligately psychrophilic bacteria isolated from Antarctica. J Gen Appl Microbiol 22:165–176 [CrossRef]
     [Google Scholar]
  12. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  13. Kämpfer P., Rainey F. A., Andersson M. A., Nurmiaho Lassila E.-L., Ulrych U., Busse H.-J., Weiss N., Mikkola R., Salkinoja-Salonen M. 2000; Frigoribacterium faeni gen. nov., sp. nov. a novel psychrophilic genus of the family Microbacteriaceae . Int J Syst Evol Microbiol 50:355–363 [CrossRef]
     [Google Scholar]
  14. Komagata K., Suzuki K.-I. 1986; Genus Curtobacterium Yamada and Komagata 1972 425AL . In Bergey's Manual of Systematic Bacteriology vol 2 pp 1313–1317 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams and Wilkins;
     [Google Scholar]
  15. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  16. Lee S. D. 2006; Phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed. Int J Syst Evol Microbiol 56:2369–2373 [CrossRef]
     [Google Scholar]
  17. Mac Faddin J. F. 1980 Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
     [Google Scholar]
  18. Manaia C. M., Nogles B., Weiss N., Nunes O. C. 2004; Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘ Brevibacterium helvolum ’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. Int J Syst Evol Microbiol 54:783–789 [CrossRef]
     [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  20. Minnikin D. E., Alshamaony L., Goodfellow M. 1977; Differentiation of Mycobacterium , Nocardia , and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88:200–204
     [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
     [Google Scholar]
  22. Park Y. H., Suzuki K., Yim D. G., Lee K. C., Kim E., Yoon J., Kim S., Kho Y. H., Goodfellow M., Komagata K. 1993; Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 94:307–313
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  24. Sheridan P. P., Loveland-Curtze J., Miteva V. I., Brenchley J. E. 2003; Rhodoglobus vestalii gen. nov., sp. nov. a novel psychrophilic organism isolated from an Antarctic Dry Valley lake. Int J Syst Evol Microbiol 53:985–994 [CrossRef]
     [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
     [Google Scholar]
  26. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
     [Google Scholar]
  27. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K. 1997; Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate “ Curtobacterium psychrophilum ” Inoue and Komagata 1976. Int J Syst Bacteriol 47:474–478 [CrossRef]
     [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
     [Google Scholar]
  29. Tiago I., Pires C., Mendes V., Morais P. V., Costa M., Verissimo A. 2005; Microcella putealis gen. nov., sp. nov., a Gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 28:479–487 [CrossRef]
     [Google Scholar]
  30. Tiago I., Morais P., Costa M., Veríssimo A. 2006; Microcella alkaliphila sp. nov., a novel member of the family Microbacteriaceae isolated from a non-saline alkaline groundwater, and emended description of the genus Microcella . Int J Syst Evol Microbiol 56:2313–2316 [CrossRef]
     [Google Scholar]
  31. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
     [Google Scholar]
  32. Yoon J. H., Kang S. J., Schumann P., Oh T. K. 2006; Yonghaparkia alkaliphila gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an alkaline soil. Int J Syst Evol Microbiol 56:2415–2420 [CrossRef]
     [Google Scholar]
  33. Zgurskaya H. I., Evtushenko L. I., Akimov V. N., Kakakoutskii L. V. 1993; Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. Int J Syst Bacteriol 43:143–149 [CrossRef]
     [Google Scholar]
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Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae
Int J Syst Evol Microbiol 57, 2498 (2007); https://doi.org/10.1099/ijs.0.64591-0
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