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Volume 63, Issue Pt_10

gen. nov., sp. nov., an anaerobic, amino-acid-degrading bacterium from soil of a Japanese rice field Free

Abstract

A novel anaerobic bacterium that could ferment amino acids and organic acids was isolated from an anaerobic, propionate-oxidizing enrichment culture originating from soil of a rice field in Japan. Cells of the isolate, designated strain 4F6E, were Gram-staining-negative, oxidase- and catalase-negative, non-spore-forming, vibrio-shaped, motile rods (0.8×2.0–2.5 µm) with two or three lateral flagella. Growth occurred at 20–42 °C (optimum at 37–40 °C), at pH 6.4–8.4 (optimum at pH 7.3) and at 0–1.5 % (w/v) NaCl (optimum at 0–0.5 %). Good growth occurred on glycine, serine, cysteine, pyruvate and citrate, whereas poor growth was observed on threonine, glutamine, -malate, α-ketoglutarate, peptone and Casamino acids. In co-culture with the hydrogen-utilizing methanogen JCM 10132, strain 4F6E oxidized alanine, valine, leucine, isoleucine, methionine, aspartate, glutamate, histidine, asparagine and fumarate. Yeast extract was required for growth. The G+C content of genomic DNA was 61.9 mol%. A phylogenetic analysis based on comparison of the 16S rRNA gene sequence showed that the type strains of , and , members of the family , were the closest relatives of strain 4F6E, with low sequence similarities (89.3, 89.5 and 86.2 %, respectively). Strain 4F6E contained iso-C (24.43 %), iso-C (16.47 %) and Cω11/Cω9 (16.32 %) as the major fatty acids, which differed from those of , and . On the basis of phenotypic, chemotaxonomic and phylogenetic differences between strain 4F6E and the type strains of and species, we propose that strain 4F6E represents a novel genus and species, gen. nov., sp. nov. The type strain of is strain 4F6E ( = JCM 18417 = DSM 25964).

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2013-10-01
2024-04-25
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Aminivibrio pyruvatiphilus gen. nov., sp. nov., an anaerobic, amino-acid-degrading bacterium from soil of a Japanese rice field
Int J Syst Evol Microbiol 63, 3679 (2013); https://doi.org/10.1099/ijs.0.052225-0
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