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Volume 62, Issue Pt_9

gen. nov., sp. nov., a member of the family , isolated from Antarctic intertidal sediment Free

Abstract

A yellow-coloured, rod-shaped, Gram-reaction- and Gram-staining-negative, non-motile and aerobic bacterium, designated strain ZS1-8, was isolated from a sample of sandy intertidal sediment collected from the Antarctic coast. Flexirubin-type pigments were absent. In phylogenetic analyses based on 16S rRNA gene sequences, strain ZS1-8 formed a distinct phyletic line and the results indicated that the novel strain should be placed in a new genus within the family . In pairwise comparisons between strain ZS1-8 and recognized species, the levels of 16S rRNA gene sequence similarity were all <93.3 %. The strain required Ca and K ions as well as NaCl for growth. Optimal growth was observed at pH 7.5–8.0, 17–19 °C and with 2–3 % (w/v) NaCl. The major fatty acids were iso-C G, iso-C, summed feature 3 (iso-C 2-OH and/or Cω7), an unknown acid with an equivalent chain-length of 13.565 and iso-C 3-OH. The major respiratory quinone was MK-6. The predominant polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 43.9 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain ZS1-8 represents a novel species in a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is ZS1-8 ( = JCM 17291 = DSM 23421).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 40876097, 41076131 and 30500001)
  • Natural Science Foundation of Shanghai (Award 11ZR1441000)
  • Ocean Public Welfare Scientific Research Project of China (Award 201005032-4)
  • Special Foundation for Comprehensive Survey of the Polar Environment (Award CHINARE2012-02-01)
© 2012 IUMS
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
     [Google Scholar]
  2. Arun A. B., Chen W. M., Lai W. A., Chao J. H., Rekha P. D., Shen F. T., Singh S., Young C. C. 2009; Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. Int J Syst Evol Microbiol 59:2738–2742 [View Article][PubMed]
     [Google Scholar]
  3. Bae S. S., Kwon K. K., Yang S. H., Lee H. S., Kim S. J., Lee J. H. 2007; Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome . Int J Syst Evol Microbiol 57:1050–1054 [View Article][PubMed]
     [Google Scholar]
  4. Barbeyron T., L’Haridon S., Corre E., Kloareg B., Potin P. 2001; Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51:985–997 [View Article][PubMed]
     [Google Scholar]
  5. Barbeyron T., Carpentier F., L’Haridon S., Schüler M., Michel G., Amann R. 2008; Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 58:790–797 [View Article][PubMed]
     [Google Scholar]
  6. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [View Article]
     [Google Scholar]
  7. Bernardet J.-F., Nakagawa Y., Holmes B. for the Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  8. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
     [Google Scholar]
  9. Bowman J. P., Nichols D. S. 2002; Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats. Int J Syst Evol Microbiol 52:1533–1541 [View Article][PubMed]
     [Google Scholar]
  10. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55:1471–1486 [View Article][PubMed]
     [Google Scholar]
  11. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [View Article][PubMed]
     [Google Scholar]
  12. Bowman J. P., McCammon S. A., Lewis T., Skerratt J. H., Brown J. L., Nichols D. S., McMeekin T. A. 1998; Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144:1601–1609 [View Article][PubMed]
     [Google Scholar]
  13. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  14. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
     [Google Scholar]
  15. Cho K. H., Hong S. G., Cho H. H., Lee Y. K., Chun J., Lee H. K. 2008; Maribacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 58:1300–1303 [View Article][PubMed]
     [Google Scholar]
  16. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  17. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  18. Felsenstein J. 2009 phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  19. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 1 pp. 119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  20. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [View Article][PubMed]
     [Google Scholar]
  21. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  22. Hwang C. Y., Kim M. H., Bae G. D., Zhang G. I., Kim Y. H., Cho B. C. 2009; Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda . Int J Syst Evol Microbiol 59:1856–1861 [View Article][PubMed]
     [Google Scholar]
  23. Jooste P. J. 1985 The taxonomy and significance of Flavobacterium–Cytophaga strains from daily sources. PhD thesis, University of the Orange Free State, Bloemfontein, South Africa.
  24. Kwon K. K., Lee Y. K., Lee H. K. 2006; Costertonia aggregata gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from a mature biofilm. Int J Syst Evol Microbiol 56:1349–1353 [View Article][PubMed]
     [Google Scholar]
  25. Lee H. S., Kwon K. K., Yang S. H., Bae S. S., Park C. H., Kim S. J., Lee J. H. 2008; Description of Croceitalea gen. nov. in the family Flavobacteriaceae with two species, Croceitalea eckloniae sp. nov. and Croceitalea dokdonensis sp. nov., isolated from the rhizosphere of the marine alga Ecklonia kurome . Int J Syst Evol Microbiol 58:2505–2510 [View Article][PubMed]
     [Google Scholar]
  26. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50:1055–1063 [View Article][PubMed]
     [Google Scholar]
  27. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  28. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  29. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Rhee M. S., Frolova G. M., Falsen E., Mikhailov V. V., Bae K. S. 2004a; Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54:1017–1023 [View Article][PubMed]
     [Google Scholar]
  30. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Lysenko A. M., Mikhailov V. V., Swings J. 2004b; Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 54:1643–1648 [View Article][PubMed]
     [Google Scholar]
  31. Nedashkovskaya O. I., Kim S. B., Lee K. H., Bae K. S., Frolova G. M., Mikhailov V. V., Kim I. S. 2005a; Pibocella ponti gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi . Int J Syst Evol Microbiol 55:177–181 [View Article][PubMed]
     [Google Scholar]
  32. Nedashkovskaya O. I., Vancanneyt M., Dawyndt P., Engelbeen K., Vandemeulebroecke K., Cleenwerck I., Hoste B., Mergaert J., Tan T. L. other authors 2005b; Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov. Int J Syst Evol Microbiol 55:1033–1038 [View Article][PubMed]
     [Google Scholar]
  33. Nedashkovskaya O. I., Vancanneyt M., De Vos P., Kim S. B., Lee M. S., Mikhailov V. V. 2007; Maribacter polysiphoniae sp. nov., isolated from a red alga. Int J Syst Evol Microbiol 57:2840–2843 [View Article][PubMed]
     [Google Scholar]
  34. Nichols C. M., Bowman J. P., Guezennec J. 2005; Olleya marilimosa gen. nov., sp. nov., an exopolysaccharide-producing marine bacterium from the family Flavobacteriaceae, isolated from the Southern Ocean. Int J Syst Evol Microbiol 55:1557–1561 [View Article][PubMed]
     [Google Scholar]
  35. Reichenbach H. 1992; Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [View Article][PubMed]
     [Google Scholar]
  36. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  37. Sohn J. H., Lee J.-H., Yi H., Chun J., Bae K. S., Ahn T.-Y., Kim S.-J. 2004; Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. Int J Syst Evol Microbiol 54:675–680 [View Article][PubMed]
     [Google Scholar]
  38. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  39. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  40. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  41. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int J Syst Evol Microbiol 54:445–448 [View Article][PubMed]
     [Google Scholar]
  42. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
     [Google Scholar]
  43. Yi H., Yoon H. I., Chun J. 2005; Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55:409–416 [View Article][PubMed]
     [Google Scholar]
  44. Yoon J. H., Kang S. J., Lee S. Y., Lee C. H., Oh T. K. 2005a; Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055 [View Article][PubMed]
     [Google Scholar]
  45. Yoon J. H., Lee M. H., Oh T. K., Park Y. H. 2005b; Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda . Int J Syst Evol Microbiol 55:1015–1019 [View Article][PubMed]
     [Google Scholar]
  46. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. 2008; Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 58:1603–1607 [View Article][PubMed]
     [Google Scholar]
  47. Yu Y., Li H., Zeng Y., Chen B. 2010; Phylogenetic diversity of culturable bacteria from Antarctic sandy intertidal sediments. Polar Biol 33:869–875 [View Article]
     [Google Scholar]
  48. Zhang G. I., Hwang C. Y., Kang S. H., Cho B. C. 2009; Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola . Int J Syst Evol Microbiol 59:1455–1459 [View Article][PubMed]
     [Google Scholar]
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Pricia antarctica gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from Antarctic intertidal sediment
Int J Syst Evol Microbiol 62, 2218 (2012); https://doi.org/10.1099/ijs.0.037515-0
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