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Volume 62, Issue Pt_6

Classification of species B as sp. nov. Free

Abstract

species B has so far only been reported from the oral cavity of dogs, cats and a ferret. In the present study, information from 15 recent isolates from different sources, including African hedgehogs (), banded mongoose (), Moholi bushbabies () and pneumonia of a cat, were compared to five strains investigated previously from bite wounds in humans inflicted by a cat and dog and from gingiva of a cat. gene sequence comparison showed that 17 isolates, including the reference strain (CCUG 19794), had identical sequences, whereas two were closely related and demonstrated 97.9 and 99.6 % similarity to strain CCUG 19794, respectively; the type strain of was the most closely related strain, with 92.3 % similarity. This is within the mean range (76–100 %) of gene sequence similarity between species of the same genus within the family . 16S rRNA gene sequencing of four strains selected based on sequence comparison showed at least 99.7 % similarity between strains of species B, with 96.2 % similarity to the type strain of the closest related species (), indicating that species B should have separate species status. Separate species status was also documented when sequence comparisons were converted to a genome similarity of 93.7 % within species B and 59.0 % to the type strain of the closest related species (). Based on analysis of the phylogenetic and phenotypic data, and since most isolates originate from the oral cavities of a diverse group of animals, it is suggested that these bacteria be classified as sp. nov.; the type strain is P683 ( = CCUG 19794 = CCM 7950 = strain 23193 = MCCM 00102), obtained from a cat. Previous reports of the type strain have shown ubiquinone-8, demethylmenaquinone-8 and menaquinone-8 as the major quinones. Polyamines in the type strain were reported as diaminopropane, putrescine, cadaverine, -norspermidine, spermidine and spermine in a previous investigation, and the major fatty acids of the type strain were reported to be C, Cω7 and C, with minor amounts of C and Cω9. The DNA G+C content of the type strain has been reported to be 40.0 mol%.

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2012-06-01
2024-04-25
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Classification of Pasteurella species B as Pasteurella oralis sp. nov.
Int J Syst Evol Microbiol 62, 1396 (2012); https://doi.org/10.1099/ijs.0.035246-0
/content/journal/ijsem/10.1099/ijs.0.035246-0
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