1887

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Volume 59, Issue 11

sp. nov., isolated from wastewater Free

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, ODN7, was isolated from a wastewater treatment plant in Korea, and its taxonomic position was investigated by use of a polyphasic taxonomic approach. Strain ODN7 grew optimally at around pH 7.5 and 30 °C and in the presence of 0–0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ODN7 fell within the cluster comprising species of the genus , clustering with C52, with which it shared highest 16S rRNA gene sequence similarity (98.9 %). The chemotaxonomic properties of strain ODN7 were consistent with those of the genus . The predominant ubiquinone was Q-10, and the major fatty acids were C 7, C 7 and/or iso-C 2-OH, and C. Major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain ODN7 was 67.2 mol%. Strain ODN7 exhibited levels of DNA–DNA relatedness of 15–32 % to the type strains of phylogenetically related species and could be differentiated from these species based on differences in phenotypic characteristics. On the basis of the data presented, strain ODN7 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ODN7 (=KCTC 22579=CCUG 57509).

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2009-11-01
2024-04-24
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References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H.(2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef] [Google Scholar]
  2. Busse, H.-J., Hauser, E. & Kämpfer, P.(2005). Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov. Int J Syst Evol Microbiol 55, 2565–2569.[CrossRef] [Google Scholar]
  3. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Huang, H.-D., Wang, W., Ma, T., Li, G.-Q., Liang, F.-L. & Liu, R.-L.(2009).Sphingomonas sanxanigenens sp. nov., isolated from soil. Int J Syst Evol Microbiol 59, 719–723.[CrossRef] [Google Scholar]
  6. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  7. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  8. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H.(2005). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef] [Google Scholar]
  9. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  10. Reddy, G. S. N. & Garcia-Pichel, F.(2007).Sphingomonas mucosissima sp. nov. and Sphingomonas desiccabilis sp. nov., from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 57, 1028–1034.[CrossRef] [Google Scholar]
  11. Romanenko, L. A., Uchino, M., Frolova, G. M., Tanaka, N., Kalinovskaya, N. I., Latyshev, N. & Mikhailov, V. V.(2007).Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity. Int J Syst Evol Microbiol 57, 358–363.[CrossRef] [Google Scholar]
  12. Romanenko, L. A., Tanaka, N., Frolova, G. M. & Mikhailov, V. V.(2009).Sphingomonas japonica sp. nov., isolated from marine crustacean Paralithodes camtschatica from the Sea of Japan. Int J Syst Evol Microbiol 59, 1179–1182.[CrossRef] [Google Scholar]
  13. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  15. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  17. Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T. & Yamamoto, H.(1990). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34, 99–119.[CrossRef] [Google Scholar]
  18. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  19. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  20. Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef] [Google Scholar]
  21. Yoon, J.-H., Lee, M.-H., Kang, S.-J., Lee, S.-Y. & Oh, T.-K.(2006).Sphingomonas dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 2165–2169.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Kang, S. J., Lee, S. Y. & Oh, T. K.(2008).Sphingomonas insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 58, 231–236.[CrossRef] [Google Scholar]
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Sphingomonas hankookensis sp. nov., isolated from wastewater
Int J Syst Evol Microbiol 59, 2788 (2009); https://doi.org/10.1099/ijs.0.008680-0
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