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Volume 146, Issue 4

Chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmas

The EMBL accession number for the sequence determined in this work is AJ245515.

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Abstract

To further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A physical map was prepared by two-dimensional reciprocal digestions using the restriction endonucleases HII, I, I and I-I. Sixteen cleavage sites were located on the map. Southern hybridizations of digested SPLL-V4 chromosomal DNA were done using random clones and PCR-amplified genes as probes. This confirmed fragment positions and located the two rRNA operons and the linked / genes encoding elongation factors G and Tu, respectively, on the physical map. An inversion of one of the rRNA operons was observed from hybridization data. Sequence analysis of one of the random clones identified a gene encoding a glucose-inhibited division protein. Digestions of the tomato big bud (TBB) phytoplasma chromosome with the same four enzymes revealed genome heterogeneity when compared to the closely related SPLL-V4, and a preliminary chromosome size for the TBB phytoplasma of 662 kb was estimated. This mapping information has revealed that significant genome diversity exists within the phytoplasmas.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 2-D, two-dimensional , genome mapping , gid gene , PFG, pulsed-field gels , phytoplasma , pulsed-field gel electrophoresis , ribosomal genes , SPLL, sweet potato little leaf , TBB, tomato big bud and WX, Western X
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2000-04-01
2024-04-26
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Chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmasThe EMBL accession number for the sequence determined in this work is AJ245515.
Microbiology 146, 893 (2000); https://doi.org/10.1099/00221287-146-4-893
/content/journal/micro/10.1099/00221287-146-4-893
/content/journal/micro/10.1099/00221287-146-4-893
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