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Volume 134, Issue 8

Molecular Cloning of the Plasmid-located Determinants for CS1 and CS2 Fimbriae of Enterotoxigenic of Serotype O6:K15:H16 of Human Origin Free

Abstract

The plasmid pCS001, isolated from an enterotoxigenic strain of , mediates expression of the CSl or CS2 and CS3 fimbrial adhesins in appropriate hosts. To characterize this further, dIII-generated DNA fragments of this plasmid were cloned into the vector plasmid BR322. A chimaera, called pCS200, which mediated expression of the CSl or CS2 fimbrial antigen but not of CS3 fimbrial antigen in appropriate host strains, was obtained. The DNA inserted into the vector sequences of plasmid pCS200 comprised dIII fragments of 4.7 kbp and 0.8 kbp. Plasmid pCS200-carrying wild-type hosts of serotype O6:K15:H 16 that expressed the CS1 or CS2 antigen also caused mannose-resistant agglutination of bovine red blood cells, suggesting that functional fimbriae were present on the bacterial surface. As previously observed with strain K 12 recipients of CS-fimbriae-associated plasmids mobilized from wild-type enterotoxigenic , K 12 recipients of the chimaeric plasmid pCS200 did not express the CS1 or CS2 fimbrial antigen. An oligonucleotide probe, synthesized on the basis of the published N-terminal amino acid sequence of the CS2 fimbrial subunit, hybridized to plasmid pCS200, indicating that the gene for the structural subunit of this fimbria resided on the plasmid.

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© Society for General Microbiology 1988
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1988-08-01
2024-04-26
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Molecular Cloning of the Plasmid-located Determinants for CS1 and CS2 Fimbriae of Enterotoxigenic Escherichia coli of Serotype O6:K15:H16 of Human Origin
Microbiology 134, 2189 (1988); https://doi.org/10.1099/00221287-134-8-2189
/content/journal/micro/10.1099/00221287-134-8-2189
/content/journal/micro/10.1099/00221287-134-8-2189
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