1887

Abstract

Growth, hydrogen production and cellulose digestion by strain CD2 were considerably greater when the culture pH was maintained at 7·0 than when the pH was not controlled. Furthermore, if the pH of the growth medium was controlled the number of viable organisms was 6-fold greater after 3 d incubation and 100-fold greater after 6 d incubation compared with equivalent cultures in which the pH was not controlled. The differences were due to the combined effect of low pH values and acetic acid accumulation. The number of viable organisms was 2- to 3-fold lower after 12 h incubation in substrate-free medium containing 40 m-acetic acid at pH 5·5 than in the same medium at pH 7·0. Addition of 90 m-acetic acid during growth in a cellobiose-containing medium lowered the growth rate by 30% and the rate of hydrogen production by 40%. Exposure of to oxygen for up to 2 h did not affect viability measurements provided that the organisms were subsequently transferred to reduced media.

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1981-07-01
2024-04-23
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References

  1. Ackman R. G. 1972; Porous polymer bead packings and formic acid vapour in the GLC of volatile free fatty acids. Journal of Chromatographic Science 10:560–565
    [Google Scholar]
  2. Capri M. G., Marais G. V. 1975; pH adjustment in anaerobic digestion. Water Research 9:307–313
    [Google Scholar]
  3. Carlsson J., Frölander F., Sundquist G. 1977; Oxygen tolerance of anaerobic bacteria isolated from necrotic dental pulps. Acta odontologica scandinavica 35:139–145
    [Google Scholar]
  4. Hentges D. J. 1967; Influence of pH on the inhibitory activity of formic and acetic acids for Shigella. Journal of Bacteriology 93:2029–2030
    [Google Scholar]
  5. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  6. Holdeman L. V., Moore W. E. C. 1975 Anaerobe Laboratory Manual, 3rd edn.. Blacksburg, Virginia: Virginia Polytechnic Institute & State University.;
    [Google Scholar]
  7. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bacteriological Reviews 14:1–49
    [Google Scholar]
  8. Khan A. W., Saddler J. N., Patel G. B., Colvin J. R., Martin S. M. 1980; Degradation of cellulose by a newly isolated mesophilic anaerobe, Bacteroidaceae family. FEMS Microbiology Letters 7:47–50
    [Google Scholar]
  9. Mccarty P. L. 1964; Anaerobic waste treatment fundamentals I. Chemistry and microbiology. Public Works 95:107–112
    [Google Scholar]
  10. Meynell G. G. 1963; Antibacterial mechanisms of the mouse gut. II. The role of Eh and volatile fatty acids in the normal gut. British Journal of Experimental Pathology 84:379–382
    [Google Scholar]
  11. Miller T. L., Wolin M. J. 1974; A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Applied Microbiology 27:985–987
    [Google Scholar]
  12. Morris J. G. 1976; Fifth Stenhouse Williams Memorial Lecture. Oxygen and the obligate anaerobe. Journal of Applied Bacteriology 40:229–244
    [Google Scholar]
  13. Patel G. B., Khan A. W., Agnew B. J., Colvin J. R. 1980; Isolation and characterization of an anaerobic, cellulolytic microorganism, Acetivibrio cellulolyticus gen. nov., sp. nov. International Journal of Systematic Bacteriology 30:179–185
    [Google Scholar]
  14. Rolfe R. D., Hentges D. J., Campbell B. J., Barett J. T. 1978; Factors related to the oxygen tolerance of anaerobic bacteria. Applied and Environmental Microbiology 36:306–313
    [Google Scholar]
  15. Saddler J. N., Khan A. W. 1980; Cellulase production by Acetivibrio cellulolyticus. Canadian Journal of Microbiology 26:760–765
    [Google Scholar]
  16. Sloneker J. H. 1971; Determination of cellulose and apparent hemicellulose in plant tissue by gas-liquid chromatography. Analytical Biochemistry 43:539–546
    [Google Scholar]
  17. Stewart C. S. 1975; Some effects of phosphate and volatile fatty acid salts on the growth of rumen bacteria. Journal of General Microbiology 89:319–326
    [Google Scholar]
  18. Tally F. P., Stewart P. R., Sutter V. L., Rosenblatt J. E. 1975; Oxygen tolerance of fresh clinical anaerobic bacteria. Journal of Clinical Microbiology 1:161–164
    [Google Scholar]
  19. Taras M. J., Greenberg A. E., Hoak R. D., Rand M. C. (editors) 1976 Standard Methods for the Examination of Water and Wastewater, 14th edn.. Washington, D.C.: American Public Health Association-Water Pollution Control Federation.;
    [Google Scholar]
  20. Van Huyssteen J. J. 1967; Gas chromatographic separation of digestor gases using porous polymers. Water Research 1:237–242
    [Google Scholar]
  21. Wimpenny J. W. T., Samah O. A. 1978; Some effects of oxygen on the growth and physiology of Selenomonas ruminantium. Journal of General Microbiology 108:329–332
    [Google Scholar]
  22. Zajic J. E., Kosaric N., Brosseau J. D. 1978; Microbial production of hydrogen. Advances in Biochemical Engineering 9:57–109
    [Google Scholar]
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