Clinical InvestigationIdentification of Collagen 1 as a Post-transcriptional Target of miR-29b in Skin Fibroblasts: Therapeutic Implication for Scar Reduction
Section snippets
Bioinformatics Prediction and Analysis
To genome-wide screen miRNA candidates targeting human collagen 1, the PicTar (4-way) and TargetScanS algorithms provided by miRGen database were initially used with default settings.10 Because of dozens of potential miRNAs estimated by each program, the overlapped targets of both programs were considered as putative candidates for further analysis to reduce possible false positives.11
Cell Cultures and Lines
Primary human dermal fibroblasts were obtained from adult skin specimens discarded from reconstructive surgery
Computational Prediction of miRNA Candidates Targeting Collagen 1
To investigate which miRNAs are potentially involved in collagen 1 biosynthesis, we first screened possible miRNA-binding sites in the 3′UTRs of col1α1 and col1α2 via a bioinformatic approach. As expected, for both collagen 1 subunits, PicTar and TargetScanS algorithms estimated dozens of miRNA candidates probably involved in collagen 1 post-transcriptional regulation. Several overlapped miRNAs, such as let-7 family, miR-196a/b, miR-29a/b/c and miR-92, were predicted as putative regulators of
DISCUSSION
In the present study, our data indicate that miR-29b is a potent post-transcriptional repressor of collagen 1 synthesis in skin fibroblasts. miR-29b modulates col1α1 and col1α2 at both mRNA and protein levels, which was probably mediated by canonical miRNA-induced post-transcriptional silencing. Moreover, decreased miR-29b abundance was found in skin scar as compared with normal skin and its transcriptional level also inversely related to levels of both collagen 1 subunits in scar tissue.
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Supported by National Natural Science Foundation of China (No. 81100737), Natural Science Foundation of Jiangsu Province (No. BK2011762) and Priority Academic Program Development of Jiangsu Higher Education Institution (No. 2011137).
The authors declare no conflicts of interest.