Abstract
Flavonoids were extracted from Salvia plebeian by the microwave method, the process was optimized by Response Surface Methodology. On the basis of single factor experiment, ethanol concentration, microwave power, extraction time, material to liquid ratio were selected as independent variables, and the extraction content of homoplantaginin was selected as response value. The optimum extraction conditions of flavonoids from S. plebeian were determined by Box-Behnken response surface analysis. The optimum extraction conditions were that the ethanol concentration was 56%, the ratio of material to liquid was 1:30 g/mL, the extraction time was 5 minutes, the extraction power was 560 W, and the extraction content of flavonoids was 2.38 mg/g.
The difference from the predicted value was 0.021%, which was not much different from the predicted value of the model, which fully verified the reliability of the model. The extraction parameters obtained by response surface method were accurate and reliable, and the extraction process was reasonable and feasible.
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