Hostname: page-component-76fb5796d-22dnz Total loading time: 0 Render date: 2024-04-27T04:55:17.724Z Has data issue: false hasContentIssue false
  • English
  • Français

Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats

Published online by Cambridge University Press:  09 March 2007

Susan A. Jebb ,
Stephen W Garland ,
Graham Jennings  and
Marinos Elia
Susan A. Jebb
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH
Stephen W Garland
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH
Graham Jennings
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH
Marinos Elia
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Dual-energy X-ray absorptiometry (DXA) is a novel, non-invasive technique for the measurement of gross body composition in small animals. In the present study the absolute accuracy of the Hologic QDR-lOOOW scanner was assessed by comparison with direct analysis in twelve rats with a range of body fat and bone mineral content (BMC) values. Fat masses measured by DXA and petroleumether extraction were significantly different (P<0·0023). The DXA technique consistently overestimated fat mass by approximately one third of the measured fatcontent. BMC derived from the measurement of Ca in asb gave a mean of 8·26 (range 1·57–15·71)g. BMC measured by DXA was not significantly different for the group as a whole. However, there was a trend for DXA to overestimate BMC in animals with low BMC and underestimate in those with higher BMC, compared with direct analysis, such that the 95% limits of agreement for the two techniques were +2·73 to −2·58g. These results suggest that the present small-animal software developed for use with currently available Hologic machines does not give an accurate measure of gross body composition compared with the results from classical direct analysis.

Keywords

Dual-energy X-ray absorptiometryBody compositionBody fat
Type
Body composition assessment
Information
British Journal of Nutrition , Volume 75 , Issue 6 , June 1996 , pp. 803 - 809
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Ammann, P., Rizzoli, R., Slosman, D. & Bonjour, J-P. (1992). Sequential and precise in vivo measurement of bone mineral density inrats using dual energy X-ray absorptiometry. Journal of Bone and Mineral Research 7,311–316CrossRefGoogle Scholar
Bland, J. M. & Altman, D. G. (1986). Statistical methods for assessing agreement between two methods of clinical measurement. Lancet i, 307–310CrossRefGoogle ScholarPubMed
Brunton, J. A., Bayley, H. S. & Atkinson, S. A. (1993).Validation and application of dual energy X-ray absorptiometry to measure bone mass and body composition in small infants. American Journal of Clinical Nutrition 58, 839–845.CrossRefGoogle ScholarPubMed
Ellis, K. J., Shypailo, R. J., Pratt, J. A. & Pond, W. G. (1994). Accuracy of dual energy X-ray absorptiometry measurements in children. American Journal of Clinical Nutrition 60, 660–665.CrossRefGoogle ScholarPubMed
Griffin, M. G., Kimble, R., Hopfer, W. & Pacifici, R. (1993). Dual energy X-ray absorptiometry of the rat: accuracy, precision and measurement of bone loss. Journal of Bone and Mineral Research 8, 795–800.CrossRefGoogle ScholarPubMed
Hagiwara, S., Lane, N., Engelke, K., Sebastian, A., Kimmel, D. B. & Genant, H. K. (1993). Precision and accuracy for rat whole-body and femur bone mineral determination with dual X-ray absorptiometry. Bone and Mineral Research 22, 57–68.CrossRefGoogle ScholarPubMed
Hamnett, F. S. (1925). A biochemical study of bone growth. III. Changes in the composition of the ash during growth. Journal of Biological Chemistry 64, 693–696.CrossRefGoogle Scholar
Jebb, S. A., Goldberg, G. R., Jennings, G. & Elia, M. (1995). Dual energy X-ray absorptiometry measurements of body composition: effects of depth and tissue thickness, including comparisons with direct analysis. Clinical Science 88, 319–324.CrossRefGoogle ScholarPubMed
Kimmel, D. B. & Wronski, T. J. (1990). Non-destructive measurement of bone mineral in femurs from ovariectomized rats. Calcified Tissue International 46, 101–110.CrossRefGoogle Scholar
Mitlak, B. H., Schoenfeld, D. & Neer, R. M. (1994). Accuracy, precision and utility of spine and whole-skeleton mineral measurements by DXA in rats. Journal of Bone and Mineral Research 9, 119–126.CrossRefGoogle ScholarPubMed
Svendson, O. L., Haarbo, J., Hassager, C. & Christiansen, C. (1993). Accuracy of measurements of body composition by dual energy X-ray absorptiometry in vivo. American Journal of Clinical Nutrition 57, 605–608.CrossRefGoogle Scholar
Tothill, P., Avenell, A., Love, J. & Reid, D. M. (1994). Comparisons between Hologic, Lunar and Norland dual energy X-ray absorptiometers and other techniques used for whole-body soft tissue measurements. European Journal of Clinical Nutrition 48, 781–794.Google ScholarPubMed