Summary
Spilanthes mauritiana DC., (Compositae), a East African medicinal herb containing pharmaceutically promising secondary metabolites, has successfully been raised in vitro. We have developed a clonal propagation protocol that uses juvenile plants as starting material. The addition of benzylaminopurine (BA) (1.0 μM) and naphthaleneacetic acid (NAA) (0.1 μM) to the culture medium resulted in maximum shooting response with minimal callusing. Shoots rooted best in vitro in MS medium supplemented with indole-3-acetic acid (IAA; 0.2 μM), and plants that had already developed roots showed better growth, with maximum survival rate, in the greenhouse after an initial hardening.
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References
Bringmann, G.; Rischer, H. In vitro propagation of alkaloid producing rare African Liana, Triphyophyllum peltatum. Plant Cell Rep. 20:591–595; 2001.
Dalziel, J. M. The uselful plants of West Tropical Africa. London, Academic Press, Crown Agents for the Colonies; 1937:206–242.
Dragendorff, D. Die Heilpflazen de verschiedenen volker and zeiten. Stuttgart: Ferdinand Enke; 1898.
Fabry, W.; Okemo, P. O.; Ansong, R. Fungistatic and fungicidal activity of east Africa medicinal plants. Mycoses 39:67–70; 1996.
Fabry, W.; Okemo, P. O.; Ansong, R. Antibacterial activity of East African plants. J. Ethnopharmacol. 60:79–84; 1998.
Jondiko, I. J. O. A mosquito larvicide in Splithathes mauritiana. Phytochemistry 25:2289–2290; 1986.
Kokwaro, J. O. Medicinal plants of East Africa, Kampala, Nairobi: East African Literature Bureau; 1976.
Moncousin, C. Rooting in vitro cuttings. In: Bajaj, Y. P. S., ed. Biotechnology of agriculture and forestry, vol. 17. Berlin: Springer Verlag; 1991:231–261.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Watt, P. M.; Brayer-Brandwijk, M. C. The medicinal and poisonous plants of Southern and Eastern Africa, 2nd edn. Edinburgh: E&S Livingstone; 1962.
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Bais, H.P., Green, J.B., Walker, T.S. et al. In vitro propagation of Spilanthes mauritiana DC., an endangered medicinal herb, through axillary bud cultures. In Vitro Cell.Dev.Biol.-Plant 38, 598–601 (2002). https://doi.org/10.1079/IVP2002345
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DOI: https://doi.org/10.1079/IVP2002345