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In vitro propagation of Cleome spinosa (Capparaceae) using explants from nursery-grown seedlings and axenic plants

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Summary

Two independent experiments were performed to establish micropropagation of Cleome spinosa from stem segments. In the first experiment, direct shoot organogenesis on hypocotyl explants from 2-mo.-old nursery-grown seedlings was obtained on Murashige and Skoog medium with different combinations of benzyladenine (BA) and 6-furfurylaminopurine, added either individually or in combination. Best proliferation rates occurred in the presence of 2.2 and 4.4 μM BA and the highest mean number of shoots was produced in response to 4.4 μM BA. In the second experiment, regeneration via direct organogenesis was also obtained from nodal and internodal segments of axenic plants cultured in the presence of BA (4.4 and 8.8 μM) in association with indole-3-acetic acid (IAA) (0.57 and 1.14 μM). Internodal explants were the most responsive on all media tested. The best mean number of shoots per explant was achieved on medium with 4.4 μM BA in association with 0.57 μM IAA. Histological studies of the globular structures formed at the apical portion of the explants revealed direct shoot regeneration and adventitious shoot differentiation from meristematic centers around the vascular bundles of the primary regenerants. All shoots elongated and rooted on MS0 medium. The acclimatization rates ranged between 70 and 84%. Plants reached to maturity and flowered 4 mo. after transfer to ex vitro conditions.

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Authors and Affiliations

  1. Laboratório de Biotecnologia de Plantas (LABPLAN), Universidade do Estado do Rio de Janeiro, Brazil

    Norma Albarello, Claudia Simões, Paula Faria Gonçalves Rosas, Tatiana Carvalho de Castro & Márcia Garcia Gianfaldoni

  2. Laboratório de Anatomia Vegetal, Universidade do Estado do Rio de Janeiro, Brazil

    Cátia Henriques Callado

  3. Laboratório de Micropropagação e Transformação de Plantas (LABMIT), Universidade do Estado do Rio de Janeiro, Brazil

    Elisabeth Mansur

Authors
  1. Norma Albarello
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  2. Claudia Simões
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  3. Paula Faria Gonçalves Rosas
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  4. Tatiana Carvalho de Castro
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  5. Márcia Garcia Gianfaldoni
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  6. Cátia Henriques Callado
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  7. Elisabeth Mansur
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Correspondence to Norma Albarello.

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Albarello, N., Simões, C., Rosas, P.F.G. et al. In vitro propagation of Cleome spinosa (Capparaceae) using explants from nursery-grown seedlings and axenic plants. In Vitro Cell.Dev.Biol.-Plant 42, 601–606 (2006). https://doi.org/10.1079/IVP2006828

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  • DOI: https://doi.org/10.1079/IVP2006828

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