Summary
Immature seeds of Garcinia indica Choiss, were exeised from immature fruits and cultured on Lloyd and McCown (1980), woody plant medium (WPM) with different combinations of auxins and cytokinins. Somatic embryos were obtained on the media supplemented with 6-benzy laminopurine (BA; 2.2–22.1 μM) alone or in combination with α-naphthalene acetic acid (NAA; 2.6 μM) with 80% frequency within a period of 2–3 wk. Subculture of embryos on medium containing BA (16.0 μM) supplemented with indole-3-acetic acid (IAA: 2.8–5.7 μM) and/or kinetin (4.6 μM) gave rise to clusters of secondary somatic embryos along with maturation of primary embryos. In subsequent subculture on hormone-free half-strength WPM, the embryo clusters germinated with an increase in the number of secondary somatic embryos. About 70% of somatic embryos germinated into complete plantlets, which were successfully established under greenhouse conditions.
Similar content being viewed by others
References
Anonymous; The wealth of India—raw materials, vol. 4 New Delhi: Council. of Scientific and Industrial Research; 1956;102.
Balaji, K.; Sairam Reddy, P.; Rajasekharan, R. Lipid biosynthesis in seed-derived embryogenic callus of kokum (Garcinia indica). J. Plant Biol. 27(3):283–289; 2000.
Bonga, J. M.; Durzan, D. J. Cell and tissue culture in forestry, vol 3 Dordrecht; Martinus Nijhoff Publishers; 1987.
Chen, S.; Wan, M.; Lok, B. N. Active constituents against HIV-1 protease from Garcinia mangostana Planta Med. 62:381–382; 1996.
Cooke, T. The flora of the Presidency of Bombay, vol. I Calcutta: Botanical Survey of India: 1967:80–83.
Daigny, G.; Paul, H.; Sangwan, R. S.; Sangwan-Norreel, B. S. Factors influencing secondary somatic embryogenesis in Malus x domestica Borkh (cv ‘Gloster 69’). Plant Cell Rep. 16:153–157: 1996.
Das Nerves, L. O.; Dougue, S. R. L.; Almeida de J. S.; Fevereiro, P. S. Repetitive somatic embryogenesis in Medicago truncutula ssp. Narbonensis and M. truncutula spp. Gaertn cv Jamalong. Plant Cell Rep. 18:398–405; 1999.
Deshpande, R. S.; Chavan, S. S.; Dhonukshe, B. L. In-vitro shoot regeneration in kokum (Garcinia indica L.). Ann. Plant Physiol. 13(1):31–34; 1999.
Goh, C.; Lakshmanan, P.; Loh, C. High frequency direct shoot bud regeneration from excised leaves of mangosteen (Garcinia mangostana L.). Plant Sci. 101:173–180; 1994.
Iwu, M. M.; Igboko, O. A.; Okunnji, C. O.; Tempest, M. S. Anti diabetic and aldose reductase activities of bioflavanones of Garcinia kola. J. Pharm. Pharmacol. 42:290–292: 1990.
Kulkarni, M.; Deodhar, M.; In vitro regeneration and hydroxycitric acid production in tissue culture of Garcinia indica Choiss. Indian J. Biotechnol. 1:301–304; 2002.
Lloyd, C.; McCown, B. Commercially feasible micropropagation of mountain laurel, Klamia latifolia by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30:421–427; 1980.
Murashige, T.; Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473–479; 1962.
Normah, M. N.; Nor-Azza, A. B.; Alidan, R. Factors affecting in vitro shoot proliferation and ex vitro estsblishment of mangosteen. Plant Cell Tiss. Org. Cult. 43;291–294; 1995.
Onay, A.; Pirine, V.; Tilkat, E.; Akturk, Z.; Yildrim, H. Somatic embryogenesis of pistachio from female flowers. J. Hort. Sci. Biotechnol. 79(6):960–969(5); 2004.
Panaia, M.; Senaratna, T.; Dixon, K. W.; Sivasithamparam, K. High frequency somatic embryogenesis of koala fern (Baloskion tetraphyllam. Restionaceae). In Vitro Cell. Dev. Biol. Plant 40(3):303–310; 2004.
Reddy, S. Y.; Prabhakar, J. V. Cocoa butter extenders from kokum (Garcinia indica) and phulwara (Madhuca butyraceae) butter. J. Ann. Oil Chem. Soc. 71:217–219; 1994.
Sharma, A. K.; Sharma, A. Chromosome techniques, theory and practice, 3rd edn Norfolk: Edn Fakenham Press, Ltd; 1980.
Suedecor, G.W.; Cochan, W. G. Statistical methods. New Dehli: Oxford and IBH, India; 1967:569–571.
Sundaram, B. M.; Copalkrishman, C.; Subramanian, S.; Shankaranarayan, D. Anti microbial activity of Garcinia mangostana. Planta Med. 4:59–60; 1983.
Te-Chato, S.; Lim, M. Plant regeneration of mangosteen via nodular callus formation. Plant Cell Tiss. Org. Cult. 59:89–93; 1999.
Watson, J. A.; Fang, M.; Lowenstein, J. M. Tricarbollylate and hydroxycitrate: substrate and inhibitions of ATP:eitrate oxaloacetate lyase. Arch. Biochem. Biophys. 135;209–217; 1969.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Thencane, S.B., Deodhar, S.R., Bhosle, S.V. et al. Repetitive somatic embryogenesis and plant regeneration in Garcinia indica choiss. In Vitro Cell.Dev.Biol.-Plant 42, 256–261 (2006). https://doi.org/10.1079/IVP2006763
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2006763