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Time course of the development of non-alcoholic hepatic steatosis in response to high-fat diet-induced obesity in rats

Published online by Cambridge University Press:  08 March 2007

Marie-Soleil Gauthier
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Roland Favier
Affiliation:
Équipe Mixte INSERM 221, Laboratoire de Bioénergétique Fondamentale et Appliquée, Université Joseph Fourier, Grenoble, France
Jean-Marc Lavoie*
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
*
*Corresponding author: Dr Jean-Marc Lavoie, fax +1 514 343 2181, email jean-marc.lavoie@umontreal.ca
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Abstract

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The aim of the study was to characterize the time course of the development of high-fat diet-induced hepatic steatosis and its relation to body fat accretion and changes in plasma lipid profile. Female Sprague–Dawley rats were high-fat fed (HF; 42%, kJ) for 1, 2, 4, 6, 12 and 16 weeks and compared to standard fed rats (SD). Data obtained from HF rats were further analysed by classifying the animals into obesity-prone and obesity-resistant. In HF rats, liver lipid content increased rapidly by approximately 200% during the first 2 weeks, decreased almost to baseline levels between weeks 2 and 6, and re-increased by 17% between weeks 6 and 16 (P<0·05). Body weight, body fat accretion, plasma leptin, NEFA and glycerol concentrations were higher in HF than in SD rats (P<0·05). These higher values were established in 2 weeks and the differences between the groups did not further enlarge from weeks 2 to 16. Obesity-prone rats depicted higher body weight and body fat accretion than obesity-resistant and SD rats. Surprisingly, however, liver lipid content was the same in obesity-prone as in obesity-resistant rats as they were both higher than in SD rats (weeks 2 and 16; P<0·05). Our data support the hypothesis that the liver acts as a systemic buffer, largely increasing its lipid content in the early stage of high-fat feeding. Our results also suggest that the development of non-alcoholic hepatic steatosis is more linked to dietary fat ingestion than to body weight gain.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2006

References

Axen, KV, Dikeakos, A & Sclafani, AHigh dietary fat promotes syndrome X in nonobese rats. J Nutr 2003 133, 22442249.CrossRefGoogle ScholarPubMed
Bahceci, M, Tuzcu, A, Akkus, M, Yaldiz, M & Ozbay, AThe effect of high-fat diet on the development of obesity and serum leptin level in rats. Eat Weight Disord 1999 4, 128132.CrossRefGoogle ScholarPubMed
Burt, AD, MacSween, RNM, Peters, TJ & Simpson, KJMcIntyre, NNonalcoholic fatty liver: causes and complications. In Oxford Textbook of Clinical Hepatology, pp. 865871. New York: Oxford University Press. 1991Google Scholar
Chalkley, SM, Hettiarachchi, M, Chisholm, DJ & Kraegen, EWLong-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats. Am J Physiol Endocrinol Metab 2002 282, E1231E1238.CrossRefGoogle Scholar
Commerford, SRPagliassotti, MJMelby, CL, Wei, Y, Gayles, EC & Hill, JOFat oxidation, lipolysis, and free fatty acid cycling in obesity-prone and obesity-resistant rats. Am J Physiol Endocrinol Metab 2000 279, E875E885.CrossRefGoogle ScholarPubMed
Day, CPPathogenesis of steatohepatitis. Best Pract Res Clin Gastroenterol 2002 16, 663678.CrossRefGoogle ScholarPubMed
Frayn, KNAdipose tissue as a buffer for daily lipid flux. Diabetologia 2002 45, 12011210.CrossRefGoogle ScholarPubMed
Frayn, KN & Maycock, PFSkeletal muscle triacylglycerol in the rat: methods for sampling and measurement, and studies of biological variability. J Lipid Res 1980 21, 139144.CrossRefGoogle ScholarPubMed
Gauthier, MS, Couturier, K, Charbonneau, A & Lavoie, JMEffects of introducing physical training in the course of a 16- week high-fat diet regimen on hepatic steatosis, adipose tissue fat accumulation, and plasma lipid profile. Int J Obes Relat Metab Disord 2004 28, 10641071.CrossRefGoogle ScholarPubMed
Gauthier, MS, Couturier, K, Latour, JG & Lavoie, JMConcurrent exercise prevents high-fat-diet-induced macrovesicular hepatic steatosis. J Appl Physiol 2003 94, 21272134.CrossRefGoogle ScholarPubMed
Ghibaudi, L, Cook, J, Farley, C, van Heek, M & Hwa, JJFat intake affects adiposity, comorbidity factors, and energy metabolism of Sprague-Dawley rats. Obes Res 2002 10, 956963.CrossRefGoogle ScholarPubMed
Gibbons, GF, Wiggins, D, Brown, AM & Hebbachi, AMSynthesis and function of hepatic very-low-density lipoprotein. Biochem Soc Trans 2004 32, 5964.CrossRefGoogle ScholarPubMed
Hill, JO, Lin, D, Yakubu, F & Peters, JCDevelopment of dietary obesity in rats: influence of amount and composition of dietary fat. Int J Obes Relat Metab Disord 1992 16, 321333.Google ScholarPubMed
Ji, H, Outterbridge, LV & Friedman, MIPhenotype-based treatment of dietary obesity: differential effects of fenofibrate in obesity- prone and obesity-resistant rats. Metabolism 2005 54, 421429.CrossRefGoogle ScholarPubMed
Kim, JK, Gavrilova, O, Chen, Y, Reitman, ML & Shulman, GIMechanism of insulin resistance in A-ZIP/F-1 fatless mice J Biol Chem 2000 275, 84568460.CrossRefGoogle ScholarPubMed
Kraegen, EW, Clark, PW, Jenkins, AB, Daley, EA, Chisholm, DJ & Storlien, LHDevelopment of muscle insulin resistance after liver insulin resistance in high-fat-fed rats. Diabetes 1991 40, 13971403.CrossRefGoogle ScholarPubMed
Krotkiewski, M & Bjorntorp, PThe effect of progesterone and of insulin administration on regional adipose tissue cellularity in the rat. Acta Physiol Scand 1976 96, 122127.CrossRefGoogle ScholarPubMed
Lauterio, TJ, Bond, JP & Ulman, EA (1994) Development and characterization of a purified diet to identify obesity-susceptible and resistant rat populations. J Nutr 124, 21722178.CrossRefGoogle ScholarPubMed
Levin, BE, Dunn-Meynell, AA, Balkan, B & Keesey, RESelective breeding for diet-induced obesity and resistance in Sprague-Dawley rats. Am J Physiol 1997 273, R725R730.Google ScholarPubMed
Lo, S, Russell, JC & Taylor, AWDetermination of glycogen in small tissue samples. J Appl Physiol 1970 28, 234236.CrossRefGoogle ScholarPubMed
Marceau, P, Biron, S, Hould, FS, Marceau, S, Simard, S, Thung, SN & Kral, JGLiver pathology and the metabolic syndrome X in severe obesity. J Clin Endocrinol Metab 1999 84, 15131517.CrossRefGoogle ScholarPubMed
Pagliassotti, MJ, Knobel, SM, Shahrokhi, KA, Manzo, AM & Hill, JOTime course of adaptation to a high-fat diet in obesityresistant and obesity-prone rats. Am J Physiol Regul Integr Comp Physiol 1994 267, R659R664.CrossRefGoogle ScholarPubMed
Pagliassotti, MJ, Pan, D, Prach, P, Koppenhafer, T, Storlien, L & Hill, JOTissue oxidative capacity, fuel stores and skeletal muscle fatty acid composition in obesity-prone and obesity-resistant rats. Obes Res 1995 3, 459464.CrossRefGoogle ScholarPubMed
Pagliassotti, MJ, Shahrokhi, KA & Hill, JOSkeletal muscle glucose metabolism in obesity-prone and obesityresistant rats. Am J Physiol Regul Integr Comp Physiol 1993 264, R1224R1228.CrossRefGoogle ScholarPubMed
Reue, K, Xu, P, Wang, XP & Slavin, BGAdipose tissue deficiency, glucose intolerance, and increased atherosclerosis result from mutation in the mouse fatty liver dystrophy (fld) gene. J Lipid Res 2000 41, 10671076.CrossRefGoogle ScholarPubMed
Saltiel, AR & Kahn, CRInsulin signalling and the regulation of glucose and lipid metabolism. Nature 2001 414, 799806.CrossRefGoogle ScholarPubMed
Samuel, VT, Liu, Z-X, Qu, X, Elder, BD, Bilz, S, Befroy, D, Romanelli, AJ & Shulman, GIMechanism of hepatic insulin resistance in non-alcoholic fatty liver disease. J Biol Chem 2004 279, 3234532353.CrossRefGoogle ScholarPubMed
Satia-Abouta, J, Patterson, RE, Schiller, RN & Kristal, AREnergy from fat is associated with obesity in U.S. men: results from the Prostate Cancer Prevention Trial. Prev Med 2002 34, 493501.CrossRefGoogle ScholarPubMed
Seppala-Lindroos, A, Vehkavaara, S, Hakkinen, AM, Goto, T, Westerbacka, J, Sovijarvi, A, Halavaara, J & Yki-Jarvinen, HFat accumulation in the liver is associated with defects in insulin suppression of glucose production and serum free fatty acids independent of obesity in normal men. J Clin Endocrinol Metab 2002 87, 30233028.CrossRefGoogle ScholarPubMed
Sniderman, AD, Cianflone, K, Arner, P, Summers, LKM & Frayn, KNThe adipocyte, fatty acid trapping, and atherogenesis. Arterioscler Thromb Vasc Biol 1998 18, 147151.CrossRefGoogle ScholarPubMed
Wang, J, Alexander, JT, Zheng, P, Yu, HJ, Dourmashkin, J & Leibowitz, SFBehavioral and endocrine traits of obesity-prone and obesity-resistant rats on macronutrient diets. Am J Physiol Endocrinol Metab 1998 274, E1057E1066.CrossRefGoogle ScholarPubMed
Westerbacka, J, Lammi, K, Hakkinen, AM, Rissanen, A, Salminen, I, Aro, A & Yki-Jarvinen, HDietary fat content modifies liver fat in overweight non-diabetic subjects. J Clin Endocrinol Metab 2005 90(5), 28042809.CrossRefGoogle Scholar