Molecular & Cellular Proteomics
ResearchChanges in the Oligodendrocyte Progenitor Cell Proteome with Ageing
Graphical Abstract
Highlights
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Quantitative proteome of neonatal, young, and aged OPCs.
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50% of the proteome is differentially expressed between neonatal and adult OPCs.
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Myelin proteins are increased, and cholesterol synthesis proteins decreased with age.
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Proteins associated with other neurodegenerative diseases are increased in aged OPCs.
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This article contains supplemental data.
Funding and additional information—This work was supported by grants from the UK Multiple Sclerosis Society (MS50), MedImmune, The Adelson Medical Research Foundation and a core support grant from the Wellcome Trust and MRC to the Wellcome-Medical Research Council Cambridge Stem Cell Institute (203151/Z/16/Z). A.G.d.l.F. was also supported by an ECTRIMS postdoctoral fellowship from July 2018. R.M.L.Q. was supported by the Brazilian Science without Borders program (grant number 2030981/2014-14-AstraZeneca/Brazil/CNPq).
Author contributions—A.G.d.l.F., R.M.Q., C.A.J., and R.J.M.F. designed research; A.G.d.l.F., R.M.Q., and J.F.C. performed research; A.G.d.l.F., R.M.Q., and R.J.M.F. contributed new reagents/analytic tools; A.G.d.l.F., R.M.Q., T.G., and C.E.M. analyzed data; A.G.d.l.F., D.E.B., D.C.F., K.S.L., and R.J.M.F. wrote the paper; A.G.d.l.F. designed the figures, Planned the experiments, Conducted experiments, collected data, Interpreted the data; R.M.Q. planned experiments, conducted experiments, collected data, interpreted data; C.E.M. edited the manuscript and figures; D.E.B. and D.C.F. interpreted data; C.A.J. edited the manuscript Planned experiments Supervised the project; K.S.L. interpreted the data Supervised the project; C.P.G. edited the manuscript Planned the experiments Supervised the project; R.J.M.F. designed the figures, Interpreted the data, Supervised the project.
Conflict of interest—Authors declare no competing interests.
Abbreviations—The abbreviations used are:
- AD
Alzheimer's disease
- b-FGF
basic fibroblast growth factor
- BSA
bovine seroalbumin
- ACN
acetonitrile
- CNS
central nervous system
- DMEM
Dulbecco's modified essential medium
- DTT
di-thiotreitol
- EAE
experimental autoimmune encephalomyelitis
- FDR
false discovery rate
- GO
Gene Ontology
- HBSS
Hank's balanced salt solution
- HD
Huntington's disease
- HRP
horse radish peroxidase
- IgM
immunoglobulin M
- KEGG
Kyoto Encyclopedia of Genes and Genomes
- LC-MS/MS
liquid chromatography-tandem mass spectrometry
- LIMMA
linear model for microarray analysis
- MS
multiple sclerosis
- MS/MS
tandem mass spectrometry
- MSA
multiple system atrophy
- MWB
Miltenyi wash buffer
- NDS
normal donkey serum
- Neo
neonatal
- OPC
oligodendrocyte progenitor cell
- PBS
phosphate buffered saline
- PC
principal component
- PCA
principal component analysis
- PD
Parkinson's disease
- PDGF-aa
platelet derived growth factor AA
- PFA
paraformaldehyde
- PSM
peptide-to-spectrum matches
- PVDF
polyvinylidene fluoride
- RNA-seq
RNA sequencing
- RT
room temperature
- TBS
tris-buffered saline
- TBST
tris-buffered saline with 0.1% Tween
- TEAB
triethylammonium bicarbonate
- TFA
trifluoroacetic acid
- TMT
tandem mass tag
- V
volts.
Present address for Alerie G. de la Fuente: Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, United Kingdom.
Present address for Clare A. Jones: Talisman Therapeutics Ltd. Cambridge, United Kingdom.