Journal of Biological Chemistry
Protein Structure and FoldingAntibiotic binding releases autoinhibition of the TipA multidrug-resistance transcriptional regulator
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Author contributions—X. J. and X. L. conceptualization; X. J. and L. Z. data curation; X. J. and L. Z. software; X. J., M. T., and X. L. supervision; X. J., L. Z., and X. L. validation; X. J. and L. Z. investigation; X. J. and X. L. writing-original draft; X. J., M. T., and X. L. project administration; X. J. and X. L. writing-review and editing; M. T. and X. L. funding acquisition; M. T. methodology; X. L. resources.
Funding and additional information—This work was supported by Chinese National Natural Science Foundation Grants 31971124, U1732114, 31770788, and 31770895 and National Key Research and Development Program of China Grant 2017YFA0503600.
Conflict of interest—The authors declare no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- MD
multidrug
- MDR
multidrug resistance
- MtaN
N terminus of Mta
- RNAP
RNA polymerase
- MerR
mercuric ion resistance
- HTH
helix-turn-helix
- ITC
isothermal titration calorimetry
- FPA
fluorescence polarization assay
- SEC
size-exclusion chromatography
- PDB
Protein Data Bank
- FAM
carboxyfluorescein
- SeMet
selenomethionine.
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These authors contributed equally to this work.