Journal of Biological Chemistry
Protein Synthesis and DegradationHeme oxygenase-2 is post-translationally regulated by heme occupancy in the catalytic site
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Author contributions—L. L., A. B. D., and S. W. R. conceptualization; L. L., A. B. D., A. S. F., and S. W. R. formal analysis; L. L. validation; L. L., A. B. D., A. S. F., and S. W. R. investigation; L. L. and S. W. R. visualization; L. L. and A. B. D. methodology; L. L. and S. W. R. writing-original draft; L. L., E. N. G. M., and S. W. R. project administration; L. L., A. B. D., A. S. F., E. N. G. M., and S. W. R. writing-review and editing; E. N. G. M. and S. W. R. supervision; S. W. R. funding acquisition.
Funding and additional information—This work was supported by National Institutes of Health Grants R01-GM123513 (to S. W. R.) and R01-GM093088 (to E. N. G. M.); and by American Heart Association Predoctoral Fellowship 19PRE34380029 (to L. L.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- 6-AN
6-aminonicotinamide
- ALAS1
d-aminolevulinic acid synthase 1
- BAF
bafilomycin
- CHX
cycloheximide
- CMA
chaperone-mediated autophagy
- ERAD
endoplasmic reticulum-associated degradation
- HD
heme-depleted
- HDX-MS
hydrogen-deuterium exchange mass spectrometry
- HO
heme oxygenase
- HRM
heme regulatory motif
- 3-MA
3-methyladenine
- SA
succinylacetone
- ER
endoplasmic reticulum
- DAPI
4′,6-diamidino-2-phenylindole
- TBST
Tris-buffered saline with Tween
- PBST
PBS containing 0.1% Tween 20.