Journal of Biological Chemistry
Cell BiologyImplications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation
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Author contributions—K. N. and N. S. conceptualization; K. N. investigation; K. N. visualization; K. N. and N. S. writing-original draft; Y. S. and S. I. resources; Y.S. and S. I. methodology; Y. S., S. I., and N. S. writing-review and editing; N. S. supervision; N. S. funding acquisition; N. S. project administration.
Funding and additional information—This work was supported by the Takeda Science Foundation and Grant-in-Aid for Scientific Research 19H03161 from the Japan Society for the Promotion of Science (JSPS) (to N. S.). This work was supported in part by the Graduate University for Advanced Studies, SOKENDAI. S. I. was supported by Grant-in-Aid for Scientific Research on Innovative Areas “nascent chain biology” JP17H05679, Grant-in-Aid for Young Scientists (A) JP17H04998, Challenging Research (Exploratory) Grant JP19K22406 from JSPS, Pioneering Projects (“Cellular Evolution”) and the Aging Project from RIKEN, and the Takeda Science Foundation. Y. S. received JSPS Research Fellowship (PD) 19J00920.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- RNG
RNA granule protein
- CHO
Chinese hamster ovary
- eIF
eukaryotic initiation factor
- eEF
eukaryotic elongation factor
- IRES
internal ribosome entry site
- more-T
more likely target transcripts
- less-T
less likely target transcripts
- CDS
coding sequence
- GO
gene ontology
- PI3K
phosphoinositide 3-kinase
- PI4K
phosphoinositide 4-kinase
- P
postnatal day
- E
embryonic day
- qRT-PCR
quantitative RT-PCR
- WGA
wheat germ agglutinin
- FBS
fetal bovine serum
- GST
glutathione S-transferase
- gRNA
guide RNA
- mWM
modified Whitten's medium
- DAPI
4′,6-diamidino-2-phenylindole
- nt
nucleotides.