Journal of Biological Chemistry
Volume 276, Issue 48, 30 November 2001, Pages 45462-45469
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GENES: STRUCTURE AND REGULATION
Transcriptional Regulation of Mouse δ-Opioid Receptor Gene: ROLE OF Ets-1 IN THE TRANSCRIPTIONAL ACTIVATION OF MOUSE δ-OPIOID RECEPTOR GENE*

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Previously, we identified a minimum core promoter of the mouse δ-opioid receptor (DOR) gene. The DOR promoter contains an E-box that binds upstream stimulatory factor and is crucial for the DOR promoter activity in NS20Y cells, a mouse neuronal cell line that constitutively expresses DOR. In the present study, we further analyzed the DOR promoter in NS20Y cells and have demonstrated that transcription factor Ets-1 binds to an Ets-1-binding site overlapping the E-box and trans-activates the DOR promoter by synergizing with upstream stimulatory factor in specific DNA binding. In addition, the Ets-1 DNA-binding domain is sufficient to play the functional role of Ets-1 in trans-activating the DOR promoter. Furthermore, through in vivo cross-linking assays and Northern blot analyses, we have demonstrated that Ets-1 binds to the DOR promoter in the neonatal mouse brain and that overexpressed Ets-1 can significantly enhance the expression of DOR mRNA in primary neonatal mouse neuronal cells. Collectively, our data suggest that Ets-1 functions as a trans-activator of the DOR promoter in the neonatal mouse brain and thus may contribute to the development of the mouse brain DOR system.

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Published, JBC Papers in Press, October 2, 2001, DOI 10.1074/jbc.M104793200

*

This work was supported by National Institutes of Health Grants DA-00546, DA-01583, DA-11806, and KO5-DA-70554 and by the A. & F. Stark Fund of the Minnesota Medical Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.