Journal of Biological Chemistry
Volume 281, Issue 17, 28 April 2006, Pages 11444-11445
Journal home page for Journal of Biological Chemistry

Accelerated Publications
Trophoblast Stem Cells Rescue Placental Defect in SOCS3-deficient Mice*

https://doi.org/10.1074/jbc.C600015200Get rights and content
Under a Creative Commons license
open access

Stem cells have important clinical and experimental potentials. Trophoblast stem (TS) cells possess the ability to differentiate into trophoblast subtypes in vitro and contribute to the trophoblast lineage in vivo. Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of cytokine signaling. Targeted disruption of SOCS3 revealed embryonic lethality on E12.5; it was caused by placental defect with enhanced leukemia inhibitory factor receptor signaling. A complementation of the wild-type (WT) placenta by using tetraploid rescue technique showed that the embryonic lethality in SOCS3-deficient embryo was due to the placental defect. Here we demonstrate that TS cells supplementation rescues placental defect in SOCS3-deficient embryos. In the rescued placenta, TS cells were integrated into the placental structure, and a substantial structural improvement was observed in the labyrinthine layer that was disrupted in the SOCS3-deficient placenta. Importantly, by supplying TS cells, living SOCS3-deficient embryos were detected at term. These results indicate a functional contribution of TS cells in the placenta and their potential application.

Cited by (0)

*

This work was supported by the Uehara Memorial Foundation (to Y. T.), a Cancer Center CORE grant (to J. N. I.), and by the American Lebanese Syrian Associated Charities (ALSAC). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

2

Present address: Dept. of Oncology-Hematology, University of Modena, Via del Pozzo 71, 41100 Modena, Italy.