Journal of Biological Chemistry
Volume 272, Issue 16, 18 April 1997, Pages 10922-10927
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CELL BIOLOGY AND METABOLISM
Association of p75NTR with Caveolin and Localization of Neurotrophin-induced Sphingomyelin Hydrolysis to Caveolae*

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Caveolae are plasma membrane microdomains that are enriched in caveolin, the structural protein of caveolae, sphingomyelin, and other signaling molecules. We previously suggested that neurotrophin-induced p75NTR-dependent sphingomyelin hydrolysis may be localized to the plasma membrane. Therefore, we examined if caveolae were a major site of p75NTR-dependent sphingomyelin hydrolysis in p75NTR-NIH 3T3 fibroblasts. Caveolin-enriched membranes (CEMs) were prepared by either detergent or detergent-free extraction and separated from noncaveolar membranes by centrifugation through sucrose gradients. Immunoblot analysis of the individual gradient fractions indicated that caveolin and p75NTR were enriched in CEMs. The localization of p75NTR to CEMs was not an artifact of receptor overexpression in the fibroblasts because a similar distribution of p75NTR was evident from PC12 cells, which endogenously express p75NTR. In the p75NTR fibroblasts, nerve growth factor induced a time-dependent hydrolysis of sphingomyelin only in CEMs with no hydrolysis detected in noncaveolar membranes. Intriguingly, endogenous p75NTR was found to co-immunoprecipitate with caveolin, suggesting that p75NTR may associate with caveolin in vivo. This interaction was confirmed in vitro by the co-immunoprecipitation of a glutathione S-transferase fusion protein expressing the cytoplasmic domain of p75NTR with caveolin. Collectively, these results demonstrate that neurotrophin-induced p75NTR-dependent sphingomyelin hydrolysis localizes to CEMs and suggest that the interaction of p75NTR with caveolin may affect signaling through p75NTR.

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*

This work was supported by Grant MCB 9513596 from the National Science Foundation (to R. T. D.) and by the Higuchi Biosciences Center.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.