Journal of Biological Chemistry
EnzymologyOxidation of cysteine 117 stimulates constitutive activation of the type Iα cGMP-dependent protein kinase
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This work was supported by National Institutes of Health Grants 5T32 HL007594-30 (to J. L. S.) and R01 HL121706 and R01 HL131181 (to M. T. N.) and additional support from the Totman Trust for Biomedical Research. The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
This article contains Figs. S1–S5.
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The abbreviations used are:
- PKG I
cGMP-dependent protein kinase I
- TCEP
tris(2-carboxyethyl)phosphine
- PDB
Protein Data Bank
- TEV
tobacco etch virus
- MWCO
molecular weight cut-off
- MeCN
acetonitrile
- Xcorr
cross-correlation.