GENES: STRUCTURE AND REGULATION
The Primary Structure of Globin and Linker Chains from the Chlorocruorin of the Polychaete Sabella spallanzanii *

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Annelid hemoglobins are organized in a very complex supramolecular network of interacting polypeptides, the structure of which is still not wholly resolved. We have separated by two-dimensional electrophoresis the 4-MDa chlorocruorin ofSabella spallanzanii and identified its components by amino-terminal sequencing. This work reveals a high rate of heterogeneity of constituent chains in a single animal as well as in the Sabella population. Using a cDNA library prepared from the hematopoietic tissue of this worm, we have isolated and fully sequenced most globin and linker cDNAs. The primary structure features of these polypeptides have been characterized by comparison with model globin and linker sequences.

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Published, JBC Papers in Press, April 6, 2001, DOI 10.1074/jbc.M006939200

*

This work was supported in part by the Italian Ministry of University and of Scientific and Technological Research MURST Grant-Cofinanziamento Protocol 9805192993-002 (to A. G.-M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequences reported in this paper have been submitted to the DDBJ/GenBankTM/EBI Data Bank with accession numbers (globin 1), (globin 2), (globin 3), (linker 1), (linker 2) and (linker 3).

§

Both authors contributed equally to this work.

**

Post-doctoral fellow of the Fund for Scientific Research-Flanders (FWO).

© 2001 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.