Journal of Biological Chemistry
Volume 276, Issue 15, 13 April 2001, Pages 11461-11464
Journal home page for Journal of Biological Chemistry

ACCELERATED PUBLICATION
An Escherichia coli Mutant Defective in Lipid Export*

https://doi.org/10.1074/jbc.C100091200Get rights and content
Under a Creative Commons license
open access

Escherichia coli phospholipids and lipopolysaccharide, made on the inner surface of the inner membrane, are rapidly transported to the outer membrane by mechanisms that are not well characterized. We now report a temperature-sensitive mutant (WD2) with an A270T substitution in a trans-membrane region of the ABC transporter MsbA. As shown by32Pi and 14C-acetate labeling, export of all major lipids to the outer membrane is inhibited by ∼90% in WD2 after 30 min at 44 °C. Transport of newly synthesized proteins is not impaired. Electron microscopy shows reduplicated inner membranes in WD2 at 44 °C, consistent with a key role for MsbA in lipid trafficking.

Cited by (0)

Published, JBC Papers in Press, February 22, 2001, DOI 10.1074/jbc.C100091200

*

This work was supported in part by National Institutes of Health Grants GM-51310 (to C. R.) and 1-F32-AI-10613-01 (to W. D.). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.