Basic and Clinical ImmunologyT cells and eosinophils cooperate in the induction of bronchial epithelial cell apoptosis in asthma☆,☆☆
Section snippets
Subjects
Approval for the use of human tissues was granted by the ethics committee of Davos. The fiberoptic bronchial biopsy specimens of 7 asthma patients (mild persistent asthma according to the Global Initiative for Asthma guidelines) were fixed in 4% paraformaldehyde and embedded in paraffin. Control bronchial mucosa was obtained from 3 patients with malignant lung tumors. T cells and eosinophils were purified from 11 atopic and 6 healthy donors.
Reagents
Human recombinant IFN-γ was a gift from Novartis
Apoptosis receptor expression and induction of apoptosis in bronchial ECs
To assess EC surface molecules, we examined ECs by using flow cytometry (Fig 1, A ).
Discussion
The present study demonstrates that ECs are targets of activated T cells and eosinophils that invade the bronchial wall in asthma. Among the growing complexity in the pathogenic mechanisms of asthma, the potential role of T cells and eosinophils is suggested. It is proposed that ECs can be the target of the immunologic injury in certain inflammatory disorders, such as eczematous dermatitis and asthma.8, 9, 18 A morphologic study of the bronchial mucosa from patients with asthma demonstrated
References (38)
Human eosinophils
J Allergy Clin Immunol
(1997)- et al.
The differential fate of cadherins during T cell-induced keratinocyte apoptosis leads to spongiosis in eczematous dermatitis
J Invest Dermatol
(2001) - et al.
A novel assay for apoptosis: flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V
J Immunol Method
(1995) - et al.
Targeting keratinocyte apoptosis in the treatment of atopic dermatitis and allergic contact dermatitis
J Allergy Clin Immunol
(2001) - et al.
Inhibition of programmed eosinophil death—a key pathogenic event for eosinophilia?
Immunol Today
(1995) - et al.
Regulated commitment of TNF receptor signaling: a molecular switch for death or activation
Immunity
(1999) Mechanisms of eosinophil-associated inflammation
J Allergy Clin Immunol
(2000)- et al.
Pulmonary T cells and eosinophils: coconspirators or independent triggers of allergic respiratory pathology
J Allergy Clin Immunol
(2001) - et al.
Targeting cytokines in asthma therapy: round one
Lancet
(2000) New directions in allergic diseases: mechanism-based anti-inflammatory therapies
J Allergy Clin Immunol
(2000)
Damage of the airway epithelium and bronchial reactivity in patients with asthma
Am Rev Respir Dis
Bronchial biopsies in asthma: an ultrastructural, quantitative study and correlation with hyperreactivity
Am Rev Respir Dis
Bronchial epithelial shedding in asthmatics and non-asthmatics
Respir Med
Identification of T lymphocytes, macrophages, and activated eosinophils in the bronchial mucosa in intrinsic asthma
Am Rev Respir Dis
Mucosal inflammation and asthma
Am J Respir Crit Care Med
Asthma
New Engl J Med
T cell-mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis
J Clin Invest
Induction and function of eosinophil intercellular adhesion molecule-1 and HLA-DR
J Immunol
Display of expression products of cDNA libraries on phage surfaces. A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction
Eur J Biochem
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Cellular and molecular mechanisms of allergic asthma
2022, Molecular Aspects of MedicineCitation Excerpt :However, the loss of epithelial integrity is also observable in chronic asthma, as cytokines and other mediators produced during type 2 inflammation can open TJs as well (Sugita et al., 2018b; Wawrzyniak et al., 2017). Moreover, T cells and eosinophils were shown to cooperate in the induction of bronchial EC apoptosis in asthma (Trautmann et al., 2002), and an increased level of apoptotic activity was found in the bronchial biopsies of patients with severe asthma (Cohen et al., 2007). Essentially, ECs can be triggered in 3 ways: injury, activation, and opening of cell-cell adhesion molecules (TJ, adherens junction).
Ceramide in apoptosis and oxidative stress in allergic inflammation and asthma
2021, Journal of Allergy and Clinical ImmunologyThe proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma
2020, Pulmonary Pharmacology and Therapeutics
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Supported by the Swiss National Foundation (32-65661-01 and 31-63381-00) and the Saurer Foundation. A.T. is a recipient of a grant from the Deutsche Forschungsgemeinschaft (TR460/1-1), P.S.-G. is a recipient of a grant for the promotion of academic young people of the University of Zurich.
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Reprint requests: A. Trautmann, MD, Swiss Institute of Allergy and Asthma Research (SIAF), Obere Strasse 22, CH-7270 Davos, Switzerland.