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Am J Perinatol
DOI: 10.1055/s-0042-1748165
Original Article

Assessment of Serum Interleukin-27 and Mean Platelet Volume in Late-Onset Neonatal Sepsis

Angie M. S. Tosson 
1   Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt
,
Dina M. T. Koptan
2   Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
,
Mohamed Kamal
3   Neonatology Division, Benha Children Hospital, Benha, Egypt
,
Marwa Abd Elhady
1   Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt
› Author Affiliations Funding None.
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Abstract

Objectives Late-onset sepsis (LOS) is a substantial contributor to morbidity and mortality among neonates. The use of nonculture-based tools for early diagnosis is an area of active investigation. Therefore, we aimed to evaluate the diagnostic value of serum interleukin-27 (IL-27) and mean platelet volume (MPV) in full-term neonates with LOS.

Study Design In this single-center, cross-sectional study, 90 full-term newborns were assigned to two equal-matched groups as follows: (1) culture-proven sepsis and (2) control groups. Clinical data and laboratory findings as complete blood pictures, including MPV, highly sensitive C-reactive protein, and blood culture results, were recorded. Moreover, IL-27 levels were measured using enzyme-linked immunosorbent assay.

Results IL-27 levels (median = 4,364 pg/mL) and MPV (mean = 12.02 ± 1.54 FL) were significantly higher in the culture-proven sepsis group than in the control group (p < 0.001). For IL-27, the optimum cut-off value for the diagnosis of LOS was 283.8 pg/mL with sensitivity and specificity of 97.8 and 100%, respectively. For MPV, the optimum cut-off value was 11.6 FL, with diagnostic sensitivity and specificity of 77.8 and 97.8%, respectively.

Conclusion IL-27 and MPV are promising markers for the diagnosis of LOS in full-term neonates. The diagnostic performance of IL-27 was superior to MPV.

Key Points

  • Late-onset neonatal sepsis diagnosis is time consuming.

  • Nonculture-based rapid diagnostic tests are much needed.

  • IL-27 is superior in LOS diagnosis to MPV.

Keywords

interleukin-27 - late-onset neonatal sepsis - mean platelet volume - nonculture-based markers


Publication History

Received: 11 December 2021

Accepted: 17 March 2022

Article published online:
17 May 2022

© 2022. Thieme. All rights reserved.

Thieme Medical Publishers, Inc.
333 Seventh Avenue, 18th Floor, New York, NY 10001, USA

 

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