Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1597037
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

DNA fingerprint, metabolomics and microscopy provide new evidence for the standardization of Armeniacae semen and Persicae semen

J Park
1   Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea
,
C Lee
1   Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea
,
S Park
1   Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea
,
YS Kim
1   Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea
,
Y Suh
1   Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

The identification of botanical origin is a prerequisite process for the regulation of botanical medicines. Armeniacae Semen and Persicae Semen are seeds of Prunus species (Rosaceae), which have been used as important traditional oriental medicine in Korea, China and Japan. Armeniacae Semen is the seed of Prunus armeniaca, P. armeniaca var. ansu or P. mandshurica var. glabra and Persicae Semen is the seed of P. persica or P. davidiana. Even if these botanical medicines have been used in the treatments of different symptoms, they are similar in shape and in chemical profiles, causing confusion in discriminating Armeniacae Semen from Persicae Semen [1, 2]. Comparative analyses empolying DNA fingerprinting, NMR-based metabolomics and microscopy were carried out to discriminate them. The molecular analyses of psbA-trnH, petN-psbM and trnS-trnG intergenic spacer of chloroplast DNA successfully traced the botanical origins of Armeniacae Semen and Persicae Semen, which are P. armeniaca and P. persica, respectively. The samples of Armeniacae Semen separated from those of Persicae Semen mainly by galactose and glutamate in NMR-based metabolomics empolying orthogonal projections to latent structures discriminant analysis. In microscopic anatomical examination, the endosperm cells were arranged in a single layer for Armeniacae Semen and the seeds of P. davidiana, whereas two or more layers for the seeds of P. persica. In result, comprehensive approach utilizing DNA fingerprint, metabolomics and microscopy work effectively for the authentication of Armeniacae Semen and Persicae Semen.

Acknowledgements: This research was supported by a grant (12172MFDS889) from Ministry of Food and Drug Safety in 2014.

Keywords: Armeniacae semen, DNA fingerprint, metabolomics, microscopy, Persicae semen.

References:

[1] Lee S, Kim Y, Kim J, Lee G, Choi G, Ju Y. Identification of Armeniacae semen and Persicae semen. Kor J Herbology 2014; 29: 61 – 67

[2] Tani T, Katsuki T, Kubo M, Arichi S. Numerical discrimination of Persicae semen and Armeniacae semen. Shoyakugaku Zasshi 1986; 40: 281 – 288