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Thromb Haemost 2000; 83(05): 732-735
DOI: 10.1055/s-0037-1613901
Review Article
Schattauer GmbH

Cloning and Characterization of the Murine Coagulation Factor X Gene

Adrian Cooper
1   From the Department of Chemistry and Biochemistry and the WM Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
,
Zhong Liang
1   From the Department of Chemistry and Biochemistry and the WM Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
,
Francis J. Castellino
1   From the Department of Chemistry and Biochemistry and the WM Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
,
Elliot D. Rosen
1   From the Department of Chemistry and Biochemistry and the WM Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
› Author AffiliationsThe authors are thankful to Melanie DeFord for technical and general assistance. This work was supported by grant HL-19982 from the National Institutes of Health, a grant from the W. M. Keck Foundation, and the Kleiderer-Pezold Family endowed professorship (to F. J. C.) and grant INN-97-216-BGIA from the American Heart Association, Indiana Affiliate (to E. D. R.).
Further Information

Publication History

Received 22 July 1999

Accepted after revision 17 December 1999

Publication Date:
08 December 2017 (online)

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Summary

The gene encoding murine coagulation factor X (fX) was isolated and characterized from a λFIX II library generated from murine genomic DNA. The 20130 bp sequence contains 18049 nucleotides that extend from the initiating methionine to the polyadenylation site. 1056 nucleotides 5’ of the start codon were determined and contain putative start sites for the FX mRNA as well as sites for binding of putative transcription factors. The sequence extends 1024 3’ of the polyadenylattion site.The gene contains 8 exons and 7 introns which were determined by comparing the mouse FX cDNA and gene sequences. The exonic structure of the gene is similar to that of the other mammalian vitamin K-dependent serine proteases of the coagulation system. These include an exon encoding the prepropepetide, the gladomain, a short helical stack, two exons for the two EGF domains, the activation pepetide, and two exons encoding the serine protease domain. The 5’ sequence of the mouse FX gene overlaps with the 3’ region of the FVII gene indicating that the murine FVII and FX gene are arranged in a head to tail arrangement as they are in humans.

Abbreviations: fVII, coagulation factor VII; fIX, coagulation factor IX; fX, coagulation factor X; PC, Protein C; fV, coagulation factor V; fVa, activated coagulation factor V; fVIII, coagulation factor VIII; fVIIIa, activated coagulation factor VIII.

Key words

Factor X - coagulation - gene - mouse - hemostasis
 

  • References

  • 1 Jackson CM, Nemerson Y. Blood coagulation. Annu Rev Biochem 1980; 49: 765-811.
    CrossrefPubMedGoogle Scholar
  • 2 Foster WB, Nesheim ME, Mann KG. The factor Xa-catalyzed activation of factor V. J Biol Chem 1983; 258: 13970-7.
    PubMedGoogle Scholar
  • 3 Radcliffe R, Nemerson Y. Activation and control of factor VII by activated factor X and thrombin. Isolation and characterization of a single chain form of factor VII. J Biol Chem 1975; 250: 388-95.
    PubMedGoogle Scholar
  • 4 Donath MJSH, Lenting PJ, VanMourik JA, Mertens K. Kinetics of factor VIII light-chain cleavage by thrombin and factor Xa. A regulatory role of the factor VIII heavy-chain region Lys713-Arg740. Eur J Biochem 1996; 240: 365-72.
    CrossrefPubMedGoogle Scholar
  • 5 Haley PE, Doyle MF, Mann KG. The activation of bovine protein C by factor Xa. J Biol Chem 1989; 264: 16303-10.
    PubMedGoogle Scholar
  • 6 Gasic GP, Arenas CP, Gasic TB, Gasic GJ. Coagulation factors X, Xa, and protein S as potent mitogens of cultured aortic smooth muscle cells. Proc Natl Acad Sci USA 1992; 89: 2317-20.
    CrossrefPubMedGoogle Scholar
  • 7 Ko FN, Yang YC, Huang SC, Ou JT. Coagulation factor Xa stimulates platelet-derived growth factor release and mitogenesis in cultured vascular smooth muscle cells of rat. J Clin Invest 1996; 98: 1493-501.
    CrossrefPubMedGoogle Scholar
  • 8 Altieri DC. Xa receptor EPR-1. FASEB J 1995; 09: 860-5.
    CrossrefPubMedGoogle Scholar
  • 9 Cirino G, Cicala C, Bucci M, Sorrentino L, Ambrosini G, DeDominicis G, Altieri DC. Factor Xa as an interface between coagulation and inflammation. Molecular mimicry of factor Xa association with effector cell protease receptor-1 induces acute inflammation in vivo. J Clin Invest 1997; 99: 2446-51.
    CrossrefPubMedGoogle Scholar
  • 10 McMullen BA, Fujikawa K, Kisiel W, Sasagawa T, Howald WN, Kwa EW, Weinstein B. Complete amino acid sequence of the light chain of human blood coagulation factor X: Evidence for identification of residue 63 as β-hydroxyaspartic acid. Biochemistry 1983; 22: 2875-84.
    CrossrefPubMedGoogle Scholar
  • 11 Leytus SP, Chung DW, Kisiel W, Kurachi K, Davie EW. Characterization of a cDNA coding for human factor X. Proc Natl Acad Sci USA 1984; 81: 3699-702.
    CrossrefPubMedGoogle Scholar
  • 12 Di Scipio RG, Hermodson MA, Davie EW. Activation of human factor X (Stuart factor) by a protease from Russell’s viper venom. Biochemistry 1977; 29: 5253-60.
    PubMedGoogle Scholar
  • 13 Hojrup P, Magnusson S. Disulphide bridges of bovine factor X. Biochem J 1987; 245: 887-91.
    CrossrefPubMedGoogle Scholar
  • 14 Titani K, Hermodson MA, Fujikawa K, Ericsson LH, Walsh KA, Neurath H, Davie EW. Bovine factor X 1a (activated Stuart factor). Evidence of homology with mammalian serine proteases. Biochemistry 1972; 11: 4899903.
    PubMedGoogle Scholar
  • 15 Nemerson Y. The reaction between bovine brain tissue factor and factors VII and X. Biochemistry 1966; 05: 601-8.
    CrossrefPubMedGoogle Scholar
  • 16 Nemerson Y. Tissue factor and hemostasis. Blood 1988; 71: 1-8.
    PubMedGoogle Scholar
  • 17 Rapaport SI. The extrinsic pathway inhibitor: A regulator of tissue factordependent blood coagulation. Thromb Haemost 1991; 66: 6-15.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 18 Broze GJJ, Girard TJ, Novotny WF. The lipoprotein-associated coagulation inhibitor. Prog Hemost Thromb 1991; 10: 243-68.
    PubMedGoogle Scholar
  • 19 Fujikawa K, Coan MH, Legaz ME, Davie EW. The mechanism of activation of bovine factor X (Stuart factor) by intrinsic and extrinsic pathways. Biochemistry 1974; 13: 5290-9.
    CrossrefPubMedGoogle Scholar
  • 20 Fujikawa K, Legaz ME, Davie EW. Bovine factor X1 (Stuart factor). Mechanism of activation by protein from Russell’s viper venom. Biochemistry 1972; 11: 4892-9.
    CrossrefPubMedGoogle Scholar
  • 21 Liang Z, Cooper A, DeFord ME, Carmeliet P, Collen D, Castellino FJ, Rosen ED. Cloning and characterization of a cDNA encoding murine coagulation factor X. Thromb Haemost 1998; Jul; 80 (01) 87-91.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Heidtmann HH, Kontermann RE. Cloning and recombinant expression of mouse coagulation factor X. Thromb Res 1998; Oct 1; 92 (01) 33-41.
    CrossrefPubMedGoogle Scholar
  • 23 Stanton C, Ross RP, Hutson S, Wallin R. Processing and expression of rat and human clotting factor-X-encoding cDNAs. Gene 1996; Mar 9; 169 (02) 269-73.
    CrossrefPubMedGoogle Scholar
  • 24 Pendurthi UR, Anderson KD, James HL. Characterization of a full-length cDNA for rabbit factor X. Thromb Res 1997; Mar 15; 85 (06) 503-14.
    CrossrefPubMedGoogle Scholar
  • 25 Boguski MS, Lowe TM, Tolstoshev CM. dbEST – database for “expressed sequence tags”. Nat Genet 1993; Aug; 04 (04) 332-3.
    CrossrefPubMedGoogle Scholar
  • 26 Benton WD, Davis RW. Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 1977; Apr 8; 196 (4286): 180-2.
    CrossrefPubMedGoogle Scholar
  • 27 Needleman SB, Wunsch CD. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 1970; Mar; 48 (03) 443-53.
    CrossrefPubMedGoogle Scholar
  • 28 Liang Z, Cooper A, Rosen ED, Castellino FJ. Chromosomal arrangement of the murine coagulation factor VII and factor X genes. Thromb Haemost 1998; Sep; 80 (03) 524-5.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 29 Gilgenkrantz S, Briquel ME, Andre E, Alexandre P, Jalbert P, Le Marec B, Pouzol P, Pommereuil M. Structural genes of coagulation facots VII and X located on 13q34. Ann Genet 1986; 29: 32-5.
    PubMedGoogle Scholar
  • 30 Wilberding J, Castellino FJ. Thromb Hemost. 1999; (August abstr.), 37.
    PubMedGoogle Scholar
  • 31 Idusogie E, Rosen ED, Carmeliet P, Collen D, Castellino FJ. Nucleotide structure and characterization of the murine blood coagulation factor VII gene. Thromb Haemost 1996; Dec; 76 (06) 957-64.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 32 Foster DC, Yoshitake S, Davie EW. The nucleotide sequence of the gene for human protein C. Proc Natl Acad Sci USA 1985; Jul; 82 (14) 4673-7.
    CrossrefPubMedGoogle Scholar
  • 33 Yoshitake S, Schach BG, Foster DC, Davie EW, Kurachi K. Nucleotide sequence of the gene for human factor IX (antihemophilic factor B). Biochemistry 1985; 24 (14) 3736-50.
    CrossrefPubMedGoogle Scholar
  • 34 Myers E, Miller W. Optimal Alignments in Linear Space. CABIOS 1988; 04: 11-7.
    PubMedGoogle Scholar
  • 35 Leytus SP, Foster DC, Kurachi K, Davie EW. Gene for Human Factor X: A Blood Coagulation Factor Whose Gene Organization is Essentially Identical with Thhat pf FIX and Protein C. Biochemistry 1986; 25: 5098-102.
    CrossrefPubMedGoogle Scholar