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Changes in nitrogen level and mycorrhizal structure of Scots pine seedlings inoculated with Thelephora terrestris

Modifications du niveau d’azote et de la structure mycorhizienne de semis de pin sylvestre inoculés par Thelephora terrestris

Annals of Forest Science volume 65, page 409 (2008)Cite this article

Abstract

  • • We investigated the influence of Thelephora terrestris, an ectomycorrhizal fungus, on the concentration of nitrogen in needles of Pinus sylvestris L. seedlings and their mycorrhizal structure within four seasons of growth.

  • • Seedlings were grown in four treatments: (I) inoculated + 0.030 g N, (II) inoculated + 0.045 g N, (III non-inoculated + 0.030 g N, (IV) non-inoculated + 0.045 g N under laboratory conditions and later planted in post-agricultural land.

  • • For inoculated treatments, statistically significant differences in N concentration of needles were observed for two- and four-year-old seedlings. The number of ectomycorrhizae and concentration of N in needles were negatively correlated. After four seasons of growth the greatest numbers of ectomycorrhizae were present on seedlings from treatment II, and the smallest on seedlings from treatment IV.

  • • Two-year-old seedlings had been colonized by at least six different fungal taxa, i.e. Cenococcum geophilum, Hebeloma crustuliniforme, Rhizopogon sp., Suillus bovinus, Thelephora terrestris and a non-identified one. Treatments I and II were dominated by the ectomycorrhizae of T. terrestris and their number was significantly greater than in treatments III or TV. On four-year-old seedlings ectomycorrhizae of T. terrestris were dominated in all treatments, while ectomycorrhizae of Hebeloma sp. were not found.

Résumé

  • • L’influence du champignon ectomycorhizien Thelephora terrestris sur la concentration en azote dans les aiguilles de semis de pin sylvestre et sur la structure mycorhizienne des plants a été suivie durant quatre saisons de croissance.

  • • Les semis ont été cultivés sous quatre traitements : (I) inoculé + 0,030 g N, (II) inoculés + 0,045 g N, (III non inoculés + 0,030 g N, (IV) non inoculés + 0,045 g N, dans les conditions du laboratoire avant d’être plantés sur le terrain.

  • • Dans les traitements d’inoculés, des différences significatives ont été observées dans la concentration des aiguilles en azote pour les semis de deux et quatre ans. Le nombre de mycorhizes et la concentration des aiguilles en azote étaient corrélés négativement. Après quatre saisons de croissance, le plus grand nombre de mycorhizes a été obtenu dans le traitement II et le plus petit dans le traitement IV

  • • Les semis de deux ans avaient été colonisés par au moins six taxons différents de champignon : Cenococcum geophilum, Hebeloma crustuliniforme, Rhizopogon sp., Suillus bovinus, Thelephora terrestris et un autre taxon indéterminé. Dans les traitements I et II, les ectomycorhizes de T. terrestris dominaient et leur nombre était plus élevé que dans les traitements III et IV Pour les semis de quatre ans, les ectomycorhizes de T. terrestris dominaient dans tous les traitements, tandis que celles de Hebeloma sp. étaient absentes.

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Authors and Affiliations

  1. Department of Forest Pathology, Forest Research Institute, Braci Lesnej 3, Sękocin Stary, 05-090, Raszyn, Poland

    Dorota Hilszczańska, Monika Małecka & Zbigniew Sierota

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  1. Dorota Hilszczańska
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  2. Monika Małecka
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  3. Zbigniew Sierota
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Correspondence to Dorota Hilszczańska.

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Hilszczańska, D., Małecka, M. & Sierota, Z. Changes in nitrogen level and mycorrhizal structure of Scots pine seedlings inoculated with Thelephora terrestris . Ann. For. Sci. 65, 409 (2008). https://doi.org/10.1051/forest:2008020

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Annals of Forest Science

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