Precisely modulating the chromatin tracker via substituent engineering: reporting pathological oxidative stress during mitosis†
a
and
Hongping
Zhou
*ab
Abstract
An in-depth understanding of cancer-cell mitosis presents unprecedented advantages for solving metastasis and proliferation of tumors, which has aroused great interest in visualizing the behavior via a luminescence tool. We developed a fluorescent molecule CBTZ-yne based on substituent engineering to acquire befitting lipophilicity and electrophilicity for anchoring lipid droplets and the nucleus, in which the low polarity environment and nucleic acids triggered a “weak–strong” fluorescence and “short–long” fluorescence-lifetime response. Meaningfully, CBTZ-yne visualized chromatin condensation, alignment, pull–push, and separation as well as lipid droplet dynamics, for the first time, precisely unveiling the asynchronous cellular mitosis processes affected by photo-generation reactive oxygen species according to the subtle change of fluorescence-lifetime. Our work suggested a new guideline for tracking the issue of the proliferation of malignant tumors in photodynamic therapy.
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