A cellular platform for production of C4 monomers

Matthew A. Davis; Vivian Yaci Yu; Beverly Fu; Miao Wen; Edward J. Koleski; Joshua Silverman; Charles A. Berdan; Daniel K. Nomura; Michelle C. Y. Chang

doi:10.1039/D3SC02773B

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

A cellular platform for production of C₄ monomers†

Matthew A. Davis,‡^a Vivian Yaci Yu,‡^a Beverly Fu,

^b Miao Wen,^b Edward J. Koleski,^b Joshua Silverman,^c Charles A. Berdan,^b Daniel K. Nomura

^abd and Michelle C. Y. Chang

*^abe

Author affiliations

* Corresponding authors

^a Department of Molecular & Cellular Biology, University of California, Berkeley, CA 94720-3200, USA
E-mail: mcchang@berkeley.edu

^b Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA

^c Calysta, 1900 Alameda de las Pulgas Suite 200, San Mateo, CA 94404, USA

^d Department of Nutritional Sciences & Toxicology, University of California, Berkeley, CA 94720-3104, USA

^e Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA 94720-1462, USA

Abstract

Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved yields of up to ∼95% for three C₄ commodity chemicals, n-butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points.

This article is part of the themed collections: Most popular 2023 chemical biology articles and 2023 Chemical Science HOT Article Collection

Download options Please wait...

Supplementary files

Supplementary information PDF (3640K)

Article information

DOI: https://doi.org/10.1039/D3SC02773B
Article type: Edge Article
Submitted: 31 May 2023
Accepted: 21 Sep 2023
First published: 09 Oct 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry

Download Citation

Chem. Sci., 2023,14, 11718-11726

Permissions

Request permissions

A cellular platform for production of C₄ monomers

M. A. Davis, V. Y. Yu, B. Fu, M. Wen, E. J. Koleski, J. Silverman, C. A. Berdan, D. K. Nomura and M. C. Y. Chang, Chem. Sci., 2023, 14, 11718 DOI: 10.1039/D3SC02773B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Science