Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions

Chuxuan Ling; Zhicheng Jin; Justin Yeung; Elany Barbosa da Silva; Yu-Ci Chang; Tengyu He; Wonjun Yim; Anthony J. O’Donoghue; Jesse V. Jokerst

doi:10.1039/D3CC04142E

Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions†

Chuxuan Ling,

^a Zhicheng Jin,

^a Justin Yeung,

^b Elany Barbosa da Silva,

^c Yu-Ci Chang,

^d Tengyu He,^d Wonjun Yim,

^d Anthony J. O’Donoghue^c and Jesse V. Jokerst

*^ade

Author affiliations

* Corresponding authors

^a Department of Nano and Chemical Engineering, University of California, San Diego, La Jolla, CA 92093, USA
E-mail: jjokerst@ucsd.edu

^b Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA

^c Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, USA

^d Program in Materials Science and Engineering, University of California, San Diego, La Jolla, CA 92093, USA

^e Department of Radiology, University of California, San Diego, La Jolla, CA 92093, USA

Abstract

We report here a colorimetric method for rapid detection of norovirus based on the valence-driven peptide-AuNP interactions. We engineered a peptide sequence named K1 with a cleavage sequence in between two lysine residues. The positively charged lysine groups aggregated the negatively charged nanoparticles leading to a purple color change. There was a red color when the cleavage sequence was digested by the Southampton norovirus 3C-like protease (SV3CP)—a protease involved in the life cycle of Human norovirus (HNV). The limit of detection was determined to be 320 nM in Tris buffer. We further show that the sensor has good performance in exhaled breath condensate, urine, and faecal matter. This research provides a potential easy and quick way to selectively detect HNV.

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DOI: https://doi.org/10.1039/D3CC04142E
Article type: Communication
Submitted: 28 Aug 2023
Accepted: 25 Sep 2023
First published: 25 Sep 2023

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Chem. Commun., 2023,59, 12459-12462

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Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions

C. Ling, Z. Jin, J. Yeung, E. B. da Silva, Y. Chang, T. He, W. Yim, A. J. O’Donoghue and J. V. Jokerst, Chem. Commun., 2023, 59, 12459 DOI: 10.1039/D3CC04142E

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Chemical Communications

Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions†

Abstract

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Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions

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