SCHEDULED MAINTENANCE
Glucuronyl C4 dehydrogenation by the radical SAM enzyme BlsE involved in blasticidin S biosynthesis†
Qi
Zhang 
*a
*a
Abstract
Here we report functional investigation of the radical S-adenosylmethionine enzyme BlsE by using cytosylglucuronamide (CGM), which is the amide analog of cytosylglucuronic acid (CGA), an intermediate involved in blasticidin S biosynthesis. We showed that, instead of decarboxylation of CGA reported previously, BlsE catalyzes C4′-dehydrogenation of CGM, and the resulting ketone is acted on by an aminotransferase BlsH to install the C4′-amino group, which uses L-Asp as the amino donor.
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