Issue 32, 2021
From the journal:

Chemical Communications

Evaluation of the role of the DNA surface for enhancing the activity of scaffolded enzymes†

Peng Lin, ORCID logo a   Huyen Dinh, ORCID logo a   Yuki Morita,a   Zhengxiao Zhang,a   Eiji Nakata,a   Masahiro Kinoshita ORCID logo a  and  Takashi Morii ORCID logo *a  

* Corresponding authors

a Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan
E-mail: t-morii@iae.kyoto-u.ac.jp

Abstract

The catalytic enhancements of enzymes loaded on DNA nanostructures have been attributed to the characteristics provided by highly negative charges on the surface of the DNA scaffold, such as the modulation of the local pH near enzymes. In this study, two types of enzymes with optimal activity at pH 6 and 8 equally displayed significant catalytic enhancements on the DNA scaffold surface. By using a ratiometric pH indicator, a lower local pH shift of 0.8 was observed near the DNA scaffold surface. The postulated local pH change near the DNA scaffold surface is unlikely to play a general role in enhancing the activity of the scaffolded enzymes.

Graphical abstract: Evaluation of the role of the DNA surface for enhancing the activity of scaffolded enzymes

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Article information

DOI
https://doi.org/10.1039/D1CC00276G
Article type
Communication
Submitted
16 Jan 2021
Accepted
14 Mar 2021
First published
15 Mar 2021

Chem. Commun., 2021,57, 3925-3928

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Evaluation of the role of the DNA surface for enhancing the activity of scaffolded enzymes

P. Lin, H. Dinh, Y. Morita, Z. Zhang, E. Nakata, M. Kinoshita and T. Morii, Chem. Commun., 2021, 57, 3925 DOI: 10.1039/D1CC00276G

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