Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis

Yanlin Wang; Qiaoyu Li; Haimei Shi; Keqi Tang; Liang Qiao; Guopeng Yu; Chuanfan Ding; Shaoning Yu

doi:10.1039/D0LC00677G

Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis†

Yanlin Wang,^a Qiaoyu Li,^a Haimei Shi,^b Keqi Tang,

^b Liang Qiao,

^a Guopeng Yu,^c Chuanfan Ding*^b and Shaoning Yu

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry, Fudan University, Shanghai 200433, China
E-mail: yushaoning@nbu.edu.cn

^b Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China
E-mail: dingchuanfan@nbu.edu.cn

^c Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Abstract

Tumor-derived exosomes, which contain RNA, DNA, and proteins, are a potentially rich non-invasive source of biomarkers. However, no efficient isolation or detection methods are yet available. Here, we developed a microfluidic Raman biochip designed to isolate and analyze exosomes in situ. Anti-CD63 magnetic nanoparticles were used to enrich exosomes through mixing channels of a staggered triangular pillar array. EpCAM-functionalized Raman-active polymeric nanomaterials (Raman beads) allow rapid analysis of exosome samples within 1 h, with a quantitative signal at 2230 cm⁻¹. The limit of detection of this biochip approaches 1.6 × 10² particles per mL with 20 μL samples. The newly developed biochip assay was successfully applied in the determination of exosomes from clinical serum samples. Thus, this novel device may have potential as a clinical exosome analysis tool for prostate cancer.

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DOI: https://doi.org/10.1039/D0LC00677G
Article type: Paper
Submitted: 02 Jul 2020
Accepted: 30 Oct 2020
First published: 05 Nov 2020

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Lab Chip, 2020,20, 4632-4637

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Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis

Y. Wang, Q. Li, H. Shi, K. Tang, L. Qiao, G. Yu, C. Ding and S. Yu, Lab Chip, 2020, 20, 4632 DOI: 10.1039/D0LC00677G

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Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis†

Abstract

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Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis

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