Issue 19, 2020
From the journal:

Lab on a Chip

Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics†

Hyungkook Jeon, ORCID logo ab   Bakr Jundi,c   Kyungyong Choi,ad   Hyunryul Ryu,a   Bruce D. Levy,c   Geunbae Lim ORCID logo b  and  Jongyoon Han ORCID logo *ade  

* Corresponding authors

a Research Laboratory of Electronics, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA
E-mail: jyhan@mit.edu

b Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Republic of Korea

c Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA

d Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA

e Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA

Abstract

A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, ∼80% WBC recovery, >85% WBC purity, and ∼12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 μL of human peripheral blood (50 μL), without any cell damage or phenotypic changes in a fully-automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments.

Graphical abstract: Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics

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DOI
https://doi.org/10.1039/D0LC00675K
Article type
Paper
Submitted
01 Jul 2020
Accepted
25 Aug 2020
First published
25 Aug 2020
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2020,20, 3612-3624

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Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics

H. Jeon, B. Jundi, K. Choi, H. Ryu, B. D. Levy, G. Lim and J. Han, Lab Chip, 2020, 20, 3612 DOI: 10.1039/D0LC00675K

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