Issue 21, 2021

Design, synthesis and cell imaging of a simple peptide-based probe for the selective detection of RNA

Abstract

Here we present a novel peptide-based fluorescent “turn-on” molecule P1 for detecting RNA, in a double or single strand, AU-rich or CG-rich. Both computational and experimental studies indicate that the detection efficiency depends on the binding affinity of P1 and conformational changes. P1 could be applied for cell imaging without any additional transfection vectors. Selective detection of RNA in cells was determined by RNase digestion. Successful application of P1 for RNA imaging in cell mitosis reveals that it may have broad applications in research, biotechnology and medical science.

Graphical abstract: Design, synthesis and cell imaging of a simple peptide-based probe for the selective detection of RNA

Supplementary files

Article information

Article type
Communication
Submitted
28 Sep 2020
Accepted
16 Jan 2021
First published
19 Jan 2021

Chem. Commun., 2021,57, 2653-2656

Design, synthesis and cell imaging of a simple peptide-based probe for the selective detection of RNA

W. Duan, F. Huang, Y. Bi, L. Zhu, D. Wang, Y. Liu, J. Wu, Y. Ge and D. Liu, Chem. Commun., 2021, 57, 2653 DOI: 10.1039/D0CC06508K

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